四川大学学报（自然科学版）2017，Vol.54Issue(2)：417-422,6.DOI:103969/j.issn.0490-6756.2017.03.033
规模化鸡场中产CMY-2大肠杆菌耐药基因与毒力基因的调查及共转移研究
Investigation and co-transfer of resistance genes and virulence genes among Escherichia coli producing CMY-2 in large-scale poultry farms
摘要
Abstract
The co-transfer of resistance genes and virulence genes among CMY-2-producing-Escherichia coli from large-scale poultry farms in China was investigated.PCR was used to examine the presence of blacMY-2 in 549 E.coli.Antimicrobial susceptibilities were tested by diffusion methods.Gene detection and co-transfer were performed for 19 resistance genes and 4 virulence genes by PCR,conjugation experiment,and PBRT.Results showed 6.0 ％ (33/549)isolates with multiple resistance carried blacMY-2 gene.15 resistance genes and 4 virulence genes were detected with high rate of floR,blaTEM-1,sul2,sul1,traT,and VagC.Co-transfer of resistance genes and virulence genes by plasmids of lncA/C or lncI1 was detected in 63.6 ％ CMY-2-positive-E.coli.This study demonstrated E.coli producing CMY-2 has become reservoirs of resistance genes and virulence genes and plasmids coharboring these two factors lead to its dissemination among large-scale poultry farms.关键词
鸡场/大肠杆菌/CMY-2/耐药基因/毒力基因/共转移Key words
Poultry farm/Escherichia coli/CMY-2/Resistance genes/Virulence gene/Co-transfer分类
农业科技引用本文复制引用
李庆周,马素贞,孔令汉,江伟,王红宁..规模化鸡场中产CMY-2大肠杆菌耐药基因与毒力基因的调查及共转移研究[J].四川大学学报（自然科学版）,2017,54(2):417-422,6.基金项目
国家蛋鸡产业技术体系岗位科学家项目(CARS-41-K09) （CARS-41-K09）
国家重点基础研究计划(2013CB127200) （2013CB127200）
