四川师范大学学报（自然科学版）2018，Vol.41Issue(1)：117-121,5.DOI:10.3969/j.issn.1001-8395.2018.01.021
腹腔神经丛阻滞对GK大鼠胰岛素受体底物的影响
The Effects of Neurolytic Celiac Plexus Block (NCPB)on Insulin Receptor Substrate in GK (Goto-Kakizaki)Rats
摘要
Abstract
To investigate the effects of neurolytic celiac plexus block (NCPB)on blood glucose and insulin receptor substrate in GK (Goto-Kakizaki)rats,the GK rats were used to establish a non-insulin dependent diabetes mellitus (NIDDM)model. 5 GK rats before NCPB were randomly selected to be subjected to the oral glucose tolerance test (OGTT)and the liver tissue was excised. The remaining 20 GK rats were randomly divided into two groups:rats in the NCPB group were treated with 0. 5% lidocaine (1mL/1 time/day)for 28 consecutive days,while the control group received the same procedure with physiological saline injected instead of 0. 5%lidocaine. The OGTT were performed after 14 and 28 days. Western blot assay was used to analyze the expression of insulin receptor substrate 1 (IRS-1),IRS-2 and phosphorylation of IRS-1 in the liver tissue. After 14 and 28 days of treatment with NCPB,the con-centration of blood glucose in the NCPB group was significantly lower than before NCPB (P<0. 05 or P<0. 05)and the control group (P<0. 01)at 0,60 and 120 min of the OGTT,while the levels of serum insulin exhibited no statistical difference between the control group and the NCPB group. The IRS-1 and IRS-2 expression levels of liver tissue in the NCPB group were significantly higher than con-trol group at the corresponding time points (P<0. 01),which significantly increased compared to before NCPB (P<0. 01). The phosphorylation level of Ser 307 of IRS-1 was significantly lower in the NCPB group than the control group (P<0. 01)at the corre-sponding time points,which declined significantly (P<0. 01)in the NCPB group,while were significantly increased (P<0. 05)in the control group compared with before NCPB. The NCPB treatment can decrease the fasting blood glucose level as well as reduce the degree of impaired glucose tolerance in GK rats. The mechanism may be related to increased protein expression and inhibition of the serine phosphorylation.关键词
腹腔神经丛阻滞/GK大鼠/口服葡萄糖耐受试验/胰岛素受体底物Key words
neurolytic celiac plexus block/Goto-Kakizaki rats/oral glucose tolerance test/insulin receptor substrate分类
医药卫生引用本文复制引用
任尧,牛杨,李军,葛方兰,杨芸,李维,杨永芝,文冬梅..腹腔神经丛阻滞对GK大鼠胰岛素受体底物的影响[J].四川师范大学学报（自然科学版）,2018,41(1):117-121,5.基金项目
国家自然科学基金面上项目(31271332)和四川省教育厅重点项目(17ZA0323) （31271332）
