水产学报2017，Vol.41Issue(12)：1838-1846,9.DOI:10.11964/jfc.20161110611
利用微卫星荧光多重PCR技术分析壳白长牡蛎3代人工选育群体的遗传多样性
Analysis of genetic diversity in mass selection lines of white-shell Pacific oyster (Crassostrea gigas) using microsatellite fluorescent multiplex PCR technique
摘要
Abstract
To investigate the genetic variances in our successive selection strains for fast growth and white shell color of the Pacific oyster (Crassostrea gigas) during mass selection process, genetic diversity of three successive mass selection strains from G1 to G3, one based stock and two wild populations was analysed using multiplex assays with ten microsatellite markers. The study showed that the average allele number (Na) ranged from 7.2 to 12.6 and the mean allelic richness (Ar) ranged from 6.8 to 11.0, the expected heterozygosity (He) and observed heterozygosity (Ho) ranged from 0.672 to 0.769 and 0.486 to 0.542, respectively. Compared to wild populations, the three successive mass selection strains showed significantly lower in the mean number of alleles and the mean allelic richness , but there was no significant difference in the average expected heterozygosity among the populations. There were 39 cases deviating significantly from Hardy-Weinberg equilibrium among the 60 population-locus cases (6 populations × 10 loci), and the inbreeding coefficient (Fis) ranged from 0.215 to 0.342. Fst values ranged from 0.005 to 0.076, showing low-moderate level genetic differentiation among the populations. The results suggest that the genetic variation has not been significantly affected by continuous mass selection and high genetic variability remains existing in the three successive selection strains, indicating that there is still potential for increased gains in future selective breeding programs.关键词
长牡蛎/壳白/微卫星/多重PCR/遗传多样性Key words
Crassostrea gigas/white-shell/microsatellite/multiplex PCR/genetic diversity分类
生物科学引用本文复制引用
邢德,李琪,张景晓..利用微卫星荧光多重PCR技术分析壳白长牡蛎3代人工选育群体的遗传多样性[J].水产学报,2017,41(12):1838-1846,9.基金项目
泰山学者种业计划专家项目(2014GB2B020029) （2014GB2B020029）
山东省科技发展计划项目(2014GHY115002)Taishan Scholars Seed Industry Experts Project Plan(2014GB2B020029) （2014GHY115002）
Science and Technology Development Project of Shandong Province (2014GHY115002) （2014GHY115002）
