水产学报2017，Vol.41Issue(12)：1878-1887,10.DOI:10.11964/jfc.20161010593
温度、流速对恩诺沙星在虹鳟体内吸收、分布的影响
Effects of temperature and water velocity on pharmacokinetics and the expression CYP3A-27 of enrofloxacin in rainbow trout(Oncorhychus mykiss)
摘要
Abstract
The pharmacokinetics of drug in animal was influenced by different environmental factors. In order to explore the effect of temperature and water velocity on pharmacokinetics of drug in rainbow trout, three temperature (5℃, 10℃, 15℃) and three water velocity (8 cm/s, 16 cm/s 24 cm/s) were studied. The temperature and water velocity were controlled by the control system, samples were determined by high-performance liquid chromatography (HPLC). Results showed that at temperatures of 5℃, 10℃ and 15℃, Tmax was 8.67 h, 4.78 h and 2.39 h; T1/2α was 0.86 h, 0.80 h and 0.77 h; T1/2β was 49.18 h, 45.81 h and 38.35 h; AUC was 140.49 μg/(L·h), 130.4 μg/(L·h) and 112.78 μg/(L·h), respectively. Metabolism of enrofloxacin in rainbow trout showed: in a certain temperature range, the rate of drug absorption and distribution at higher temperature is greater than that at lower temperature; the time to peak was shorter at higher temperature. At water velocity of 8 cm/s, 16 cm/s and 24 cm/s, Tmax was 8.57 h, 6.03 h and 4.04 h; T1/2α was 5.47 h, 2.16 h and 0.27 h; T1/2β was 26.54 h, 6.93 h and 2.13 h;T1/2ka was 7.68 h, 5.00 h and 2.01 h. The rate of drug absorption and distribution at higher water velocity is greater than that at lower water velocity; the time to peak was shorter at higher water velocity. In conclusion, the results from the present study indicated that in a certain range, the increase of temperature and water velocity improved the rate of drug absorption and distribution of enrofloxacin in rainbow trout.关键词
虹鳟/恩诺沙星/温度/流速Key words
Oncorhychus mykiss/enrofloxacin/temperature/water velocity分类
农业科技引用本文复制引用
潘浩,王荻,卢彤岩..温度、流速对恩诺沙星在虹鳟体内吸收、分布的影响[J].水产学报,2017,41(12):1878-1887,10.基金项目
公益性行业(农业)科研专项(20120385) （农业）
现代农业产业技术体系建设专项(CARS-46)Special Fund for Agri-scientific Research in the Public Interest(20120385) （CARS-46）
Special Funds of National Modern Agricultural Industry Technology System(CARS-46) （CARS-46）
