山东中医杂志2017，Vol.36Issue(12)：1063-1066,4.
补肾活血通腑方对慢性脑供血不足模型大鼠SIRT1信号通路下蛋白表达的影响
Effect of Bushen Huoxue Tongfu Formula on Protein Expression in SIRT1 Signaling Pathway in Rat Model of Chronic Cerebral Blood Deficiency
摘要
Abstract
Objective:To study the protective effect of Bushen Huoxue Tongfu formula(BHTF)on brain tissue of rats with chronic cerebral circulatory insufficiency(CCCI). Methods:Fifty healthy male Wistar rats were randomly divided into five groups:sham operation group,model group,nimodipine group,BHTF of low dose group,BHTF of high dose group. The CCCI rat model was established using 2-VO permanent ligation. After 8 weeks of intragastric administration,the rats were sacrificed through decapitation,and the hippocampus was harvested. The protein was detected by Western blot. Results:Compared with the sham group,the expression of PGC-1α and FOXO3α in the frontal lobe of the model group and other treatment groups were decreased significantly(P<0.05). Compared with the model group,the expression of PGC-1α protein in the hippocampus of the BHTF of low dose group was significantly higher than that in the model group(P<0.05). Compared with the nimodipine group,FOXO3α,PGC-1α protein expressions were significantly increased(P<0.05). Con-clusion:Cerebral ischemia in CCCI rats can inhibit the expressions of FOXO3α and PGC-1α proteins. BHTF of low dose can improve the expressions of FOXO3α and PGC-1α proteins. Increase the blood supply of the brain,thereby protecting the brain tissue of CCCI rats.关键词
慢性脑供血不足/补肾活血通腑方/大鼠Key words
chronic cerebral circulatory insufficiency/Bushen Huoxue Tongfu formula/rat分类
医药卫生引用本文复制引用
杨海昊,刘明君,韩涛,黄海量,刘西建,吕征..补肾活血通腑方对慢性脑供血不足模型大鼠SIRT1信号通路下蛋白表达的影响[J].山东中医杂志,2017,36(12):1063-1066,4.基金项目
山东省自然科学基金培养基金项目(编号:ZR2014HP060) （编号:ZR2014HP060）
