山东医药2017，Vol.57Issue(45)：13-15,3.DOI:10.3969/j.issn.1002-266X.2017.45.004
铜绿假单胞菌注射液对肺癌细胞增殖、凋亡的影响及机制
Effects of Pseudomonas aeruginosa injection on proliferation and apoptosis of lung cancer cells
摘要
Abstract
Objective To explore the effects of pseudomonas aeruginosa (PA)injection on the cell proliferation and apoptosis of lung cancer cells,and to explore its mechanism. Methods NCI-H1395 and 95-D of lung cancer cell lines were selected and then were inoculated in 96-well plate. We divided them into the control group and observation groups 1, 2,and 3,which were separately added with 0,0. 25 × 109 ,0. 5 × 109 ,and 1 × 109 / mL PA injection for 24 h. In addi-tion,the control group,PA group,and AG1478 group were set. The drug was not added into the control group,1 × 109 PA was added into the PA group,and 1 × 109 / mL PA and 10 - 7 mol/ L EGFR inhibitor AG1478 were added into the AG1478 group. The proliferation of cells was observed by MTT. The apoptosis rate of each group was detected by the AnnexinV/ PI double dye test. Results With the increase of PA concentration,the OD 570 of NCI-H1395 and 95-D cells was decreasing, and the cell apoptosis rate increased (all P < 0. 05). Compared with the control group,the OD 570 of PA group decreased (P < 0. 05),while the apoptosis rate increased (P < 0. 05). Compared with the PA group,the OD 570 of AG1478 in-creased,and the apoptosis rate decreased (both P < 0. 05). Conclusion PA injection can inhibit the proliferation of NCI-H1395 and 95-D of lung cancer cells and promote apoptosis through EGFR signaling pathway.关键词
肺肿瘤/铜绿假单胞菌注射液/细胞增殖/细胞凋亡/表皮生长因子受体Key words
lung neoplasms/Pseudomonas aeruginosa injection/cell proliferation/apoptosis/epidermal growth factor receptor分类
医药卫生引用本文复制引用
鄢俊,钟志宏,施华球..铜绿假单胞菌注射液对肺癌细胞增殖、凋亡的影响及机制[J].山东医药,2017,57(45):13-15,3.基金项目
江西省卫生厅科技计划课题(20143129). （20143129）
