山东医药2017，Vol.57Issue(45)：24-27,4.DOI:10.3969/j.issn.1002-266X.2017.45.007
不同剂量X线照射对大鼠卵巢功能的影响
Effects of different X-ray doses on the ovarian function of rats
摘要
Abstract
Objective To observe the effects of different X-ray doses on the ovarian function of rats. Methods Sixty Wistar rats were randomly divided into four groups :the control group,G0. 1 group,G1. 0 group and G10. 0 group,with different X-ray doses (0,0. 1,1. 0,and 10. 0 Gy). At 1 h,5 d (an estrous cycle),10 d (two estrous cycles)after expo-sure,the expression levels of the Bcl-2 protein,the E 2 ,FSH,and LH in the ovarian granulosa cells of Wistar rats in dif-ferent animal cycles after different X-ray doses were observed by immunohistochemistry and immunoradiometry,and then we compared the above indicators. Results At 1 h after X-ray radiation,no significant difference was found in the number of follicles and the level of each hormone between groups (all P > 0. 05). At 5 d after X-ray radiation,the number of follicles increased,the serum FSH decreased and the levels of LH and E 2 increased in the G0. 1 group as compared with those of the control group;the number of follicles decreased,the serum FSH increased and the levels of LH and E 2 decreased in the G1. 0 and G10. 0 groups (all P < 0. 05). At 10 d after X-ray radiation,the number of follicles at all levels decreased,ser-um FSH increased,and LH and E 2 decreased in the G10. 0 group (all P < 0. 05). Compared with the other groups,the number of follicles decreased,FSH increased,and LH and E 2 decreased in the G10. 0 group at 5 and 10 d after X-ray radi-ation (all P < 0. 05). At 5 d after X-ray radiation,the number of follicles increased,the serum FSH decreased,and LH and E2 increased in the G0. 1 group as compared with that at 1 h (all P < 0. 05),and they all returned to the normal levels at 10 d (P < 0. 05);at 5 d after X-ray radiation,the number of follicles decreased,the serum FSH increased,and LH and E2 decreased in the G1. 0 group as compared with that at 1 h (all P < 0. 05),and they all returned to the normal levels at 10 d (P < 0. 05);at 5 and 10 d after X-ray radiation,the number of follicles decreased,the serum FSH increased,and LH and E2 decreased in the G10. 0 group (all P < 0. 05). The positive rate of Bcl-2 in the G0. 1 group increased at 5 d af-ter X-ray radiation,decreased in the G1. 0 group at 5 d after X-ray radiation,and decreased in the G10. 0 group at 5 and 10 d after X-ray radiation (all P < 0. 05). Conclusions Low-dose X-ray radiation can inhibit the apoptosis to a certain ex-tent,and avoid the ovarian injury. When the radiation dose exceeds a certain value (greater than 1. 0 Gy),the ovarian function is impaired,but the damage is reversible. When the oversize dose (10. 0 Gy)is given,the ovarian function is se-verely damaged,even with irreversible premature aging.关键词
卵巢功能/X线电离辐射/X线辐射剂量/卵泡/细胞凋亡/生殖激素/大鼠Key words
ovarian function/ionizing radiation/follicles/apoptosis/reproductive hormone/rat分类
医药卫生引用本文复制引用
王雪芹,张永峰,刘淑华,张淑娴,徐爱霞..不同剂量X线照射对大鼠卵巢功能的影响[J].山东医药,2017,57(45):24-27,4.基金项目
山东省医药卫生科技发展基金项目(2013WS0294) （2013WS0294）
潍坊市科技局软科学基金项目(2012RKX006). （2012RKX006）
