摘要
Abstract
Objective To observe the effects of Maresin1 on the inflammation of mouse glomerular mesangial cells (GMCs)stimulated by high glucose,and its inhibitory effect on the NLR family pyrin domain-containing 3 (NLRP3)in-flammasome activation. Methods We cultured GMCs and categorized as follows:normal control group (NC group)was cultured in low glucose DMEM medium,osmotic pressure control group (OP group)was added with mannitol in low glu-cose DMEM medium,high glucose group (HG group)was cultured in high glucose DMEM medium and Maresin 1 inter-vention groups (M1 + HG group,M2 + HG group,and M3 + HG group)were treated with different concentrations of Ma-resin 1 (1,10,and 100 nmol/ L)for 30 min before being exposed to high glucose,M3 + NC group was treated with 100 nmol/ L maresin 1 for 30 min before being exposed to high glucose;meanwhile,the N-acetylcysteine (NAC)intervention group (NAC + HG group)was taken as a positive control of reactive oxygen species inhibition. We used spectrophotometer and fluorescence microscopy to detect the level of ROS. RT-PCR was used to detect the mRNA expression of NLRP3, Caspase-1,and IL-1β. Western blotting was used to detect the protein expression of NLRP3,pro-Caspase-1,Caspase-1, pro-IL-1β,and IL-1β. Results Compared with the NC group,the ROS level and the protein and mRNA expression levels of NLRP3,Caspase-1,and IL-1β increased,but the protein expression levels of pro-Caspase-1 and pro-IL-1β decreased in the HG group (all P < 0. 05). Compared with the HG group,the ROS level and the protein and mRNA expression levels of NLRP3,Caspase-1,and IL-1β decreased,but the protein expression of pro-Caspase-1 and pro-IL-1β increased in the M1+ HG group,M2 + HG group,and M3 + HG group,especially in the M3 + HG group (all P < 0. 05). Compared with the HG group,the protein and mRNA expression levels of NLRP3,Caspase-1,and IL-1β decreased but the expression of pro-Caspase-1 and pro-IL-1β increased in the M3 + HG group (all P < 0. 05). Conclusion Maresin 1 can inhibit the oxida-tive stress and inflammatory response of GMCs stimulated by high glucose,and its mechanism may be related to the de-crease of ROS level,inhibition of NLRP3 inflammasome and its related inflammatory factors.关键词
Maresin1/糖尿病肾病/炎症反应/氧化应激/肾小球系膜细胞/炎症小体Key words
Maresin 1/diabetic nephropathy/inflammatory response/oxidative stress/glomerular mesangial cells/inflammasome分类
医药卫生
