山东医药2017，Vol.57Issue(46)：27-30,4.DOI:10.3969/j.issn.1002-266X.2017.46.007
冷应激对高血压大鼠交感神经活性及脑干基质交感分子1表达的影响
Effects of cold stress on sympathetic nerve activity and Stim1 expression of rats with hypertension
摘要
Abstract
Objective To investigate the effects of cold stress on the sympathetic nerve activity and Stim1 expression of spontaneously hypertensive rats (SHR). Methods Ten male SHR and 10 normal male rats were selected. The normal male rats were randomly divided into the normal control group and cold stress group,and SHR were randomly divided into the SHR model group and SHR cold stress group,with 5 in each. Rats in the normal control group and SHR model group were placed into metabolic cages at room temperature for 6 h,and the rats in the cold stress group and SHR cold stress group were placed into metabolic cages at 4 ℃ for 6 h. Systolic (SBP)and diastolic (DBP)blood pressure was monitored during room temperature and cold stress by tail-cuff method. Urine norepinephrine excretion was detected by HPLC. The mRNA and protein expression of Stim1 in brainstem were detected by quantitative real-time PCR and Western blotting,re-spectively. Results The SBP,DBP and urinary NE excretion were significantly higher in the cold stress group than in the normal control group;the SBP,DBP,and urinary NE excretion were significantly higher in the SHR cold stress group than in the SHR model group and cold stress group. The Stim1 mRNA and protein expression levels in brainstem were signifi-cantly lower in the SHR cold stress group than in the SHR model group and cold stress group (all P < 0. 01). Conclusion In response to cold stress,Stim1 may be the key gene to modulate the sympathetic nerve activity.关键词
原发性高血压/基质交感分子1/冷应激/交感神经活性Key words
primary hypertension/stromal interaction molecule 1/cold stress/sympathetic nerve activity分类
医药卫生引用本文复制引用
肖冰,杨秀春,鲁静朝,陈飞,裴玮娜,王晓昭,刘凡..冷应激对高血压大鼠交感神经活性及脑干基质交感分子1表达的影响[J].山东医药,2017,57(46):27-30,4.基金项目
河北省医学科学研究课题(20130171) （20130171）
河北省自然科学基金青年科学基金资助项目(H2015206442) （H2015206442）
河北省引进留学人员资助项目(C201400335). （C201400335）
