陕西中医2017，Vol.38Issue(12)：1788-1790,3.DOI:10.3969/j.issn.1000-7369.2017.12.063
嗅三针对阿尔茨海默病小鼠海马区磷酸化P38MAPK和TNF-α表达的影响
Influence of Xiusanzhen on expression of phosphorylated P38MARK and TNF-α in hippocamus of mice with alzheimer's disease
摘要
Abstract
Objecticve :To observe the intervening effect of Xiusanzhen Electroacupuncture(EA) on phospho-rylation of P38MAPK and TNF-α expression in hippocampus of mice with Alzheimer's disease(AD) ,in order to clarify its therapeutic mechanism based on the olfactory nerve pathway in alleviating AD .Methods :40 AD mice were randomly devided into 4 groups :AD model group ,Xiusanzhen EA with olfactory nerve transected group (ONT+EA) ,Xiusanzhen EA group ,Donepezil HCl group ,with 10 mice respectively in each group ,then took 10 nega-tive mice as normal control group .Electroacupuncture intervention of Xiusanzhen was applied to stimulate bilateral LI 20 and EX-HN3 for 10min per time ,intragastric administration of donepezil HCI in AD mice was performed at the dose of 3mg(kg · d) ,once daily intervention (except weekends) for 4 weeks in all .Western blot technique was applied to detect p-p38MAPK protein expression in hippocampus ,immunohistochemical method was used to detect the expression level of TNF-α in hippocampus .Results Compared with AD model group ,Xiusanzhen EA group Donepezil HCl group could reduce p-p38MAPK protein and TNF-α expression in hippocampus of AD mice ,there were significant differences between the two groups (P<0 .05) .There were no significant differences were found be-tween AD model and ONT+EA groups in influencing p-p38MAPK protein and TNF-αexpression in hippocampus of AD mice (P>0 .05) .There were no significant difference between Xiusanzhen EA group and donepezil HCL group (P>0 .05) .Conclusion:Xiusanzhen EA can regulate expression of TNF-αthrough p38MAPK signaling pathway ,the integrity of the olfactory pathway is the core mechanism of the intervention effect .关键词
阿尔茨海默病/针灸疗法/@嗅三针/动物实验/小鼠Key words
Alzheimer disease/acupuncture-moxibustion/@Xiusanzhen Animal experimentation/Mice分类
医药卫生引用本文复制引用
王渊,刘智斌,牛文民,王强,李杰,鲁刚..嗅三针对阿尔茨海默病小鼠海马区磷酸化P38MAPK和TNF-α表达的影响[J].陕西中医,2017,38(12):1788-1790,3.基金项目
国家自然科学基金资助项目(81503667)陕西中医药大学重点培育科研基金(2015PY07) （81503667）
