生物技术通报2017，Vol.33Issue(11)：92-95,4.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0454
蛋白激发子PeaT1与井冈霉素混合使用诱导烟草对TMV抗性的研究
Research On the Induced Resistance of Protein Elicitor PeaT1 with Jinggangmycin in Tobacco Against TMV
摘要
Abstract
Tobacco virus infects tobacco,tomatoes,peppers,potatoes,and other crops,causing a variety of crop diseases that are difficult to control during production.To explore the optimal mixed proportion of protein elicitor PeaT1 and Jinggangmycin to defense tobacco virus disease,this study chosen Nicotiana benthamiana cv.Huangmiaoyu having 3 to 7 leaves,respectively by spraying water,Jinggangmycin,and combination of PeaT1 and PeaT1-Jinggangmycin.Virus TMV-GFP was inoculated by the way of friction,and viral infection situation was observed under UV light.The results showed that the combination of Jinggangmycin mixed with PeaT1 had better synergism than using single one;PeaT1:Jinggangmycin at 3:4 mixed ratio had the most cost-efficient,and cotoxicity coefficient reached 157.64,the relative control effect reached 73.39％.Therefore,we thought that Jinggangmycin and PeaT1 both generated tobacco resistance against TMV,and their combination had synergetic effect.关键词
蛋白激发子/井冈霉素/诱导抗性/TMV-GFPKey words
protein elicitor/Jinggangmycin/induced resistance/TMV-GFP引用本文复制引用
彭智超,仝赞华,邱德文..蛋白激发子PeaT1与井冈霉素混合使用诱导烟草对TMV抗性的研究[J].生物技术通报,2017,33(11):92-95,4.基金项目
北京市科技计划项目(D151100003915003) （D151100003915003）
