生物技术通报2017，Vol.33Issue(11)：96-100,5.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0466
甘草悬浮细胞肉桂酸-4-羟基化酶(C4H)基因的克隆及表达分析
Cloning and Expression Analysis of Cinnamate 4-hydroxylase Gene from Glycyrrhiza uralensis Suspension Cultured Cells
摘要
Abstract
According to the reported C4H (cinnamate 4-hydroxylase) gene sequence (GenBank accession ID:D87520.1) from Glycyrrhiza echinata,the coding sequence of C4H gene from Glycyrrhiza uralensis Fisch suspension cultured cells was obtained by RT-PCR cloning technology,and the bioinformatics analysis on it was carried out.In addition,the expression patterns of C4H gene induced by methyl jasmonate (MeJA) were analyzed by q-PCR.The length of the cloned C4H's open reading frame (ORF) was 1 515 bp,encoding 504 amino acids.The q-PCR experiments showed that C4H gene was induced by MeJA and reached the highest expression level at 12 h after MeJA added into the cell suspension system.关键词
肉桂酸-4-羟化酶/甘草细胞/茉莉酸甲酯/表达分析Key words
cinnamate 4-hydroxylase/licorice cells/methyl jasmonate/expression analysis引用本文复制引用
邹广平,李雅丽,冯梦薇,杨修,许智伟..甘草悬浮细胞肉桂酸-4-羟基化酶(C4H)基因的克隆及表达分析[J].生物技术通报,2017,33(11):96-100,5.基金项目
国家自然科学基金项目(31460064),内蒙古自然科学基金项目(2017MS(LH) 0304),内蒙古高等学校“青年科技英才计划”(NJYT-15-A08),内蒙古科技大学科研仪器专项(2015KYYQ03) （31460064）
