生物技术通报2017，Vol.33Issue(11)：112-122,11.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0370
利用PCR技术快速检测根际产ACC脱氨酶细菌
PCR-based Method for the Rapid Detection of ACC Deaminase-producing Rhizosphere Bacteria
摘要
Abstract
Producing ACC (1-aminocyclopropane-l-carboxylate) deaminase to reduce ethylene levels and eliminate salt stress is the key mechanism of plant-growth-promoting rhizobacteria (PGPR) in improving plant growth and stress tolerance.This work aims to establish an expeditious approach to screen bacteria containing ACC deaminase based on polymerase chain reaction (PCR).We set the acdS gene encoding the ACC deaminase as the probe,then selected three pairs of primers (acdSf3/acdSr4,DegACCf/DegACCr and F1936f/F1938r)respectively,and detected the various bacterial strains isolated from the root and rhizosphere soil of diverse halophytes and Populus deltoids.The results showed that single specific amplified bands were produced and the rate of amplification was high when using the primers acdSf3/acdSr4 combined with touch-down PCR methods;however,DegACCf/DegACCr or F1936f/F1938r was in poor specificity.Finally,we acquired 25 strains containing acds gene from 257 bacterial isolates,aiming at laying a foundation for reserving rich and functional microbial resources and further studying the genetic diversity and function of acdS in PGPR.关键词
盐生植物/杨树/植物促生长根际细菌/内生细菌/产ACC脱氨酶细菌Key words
halophytes/poplar/plant growth promoting rhizobacteria/endophytic bacteria/ACC deaminase-producing bacterium引用本文复制引用
秦媛,潘雪玉,袁志林..利用PCR技术快速检测根际产ACC脱氨酶细菌[J].生物技术通报,2017,33(11):112-122,11.基金项目
国家自然科学基金面上项目(31370704),2014年度国家林业局引智项目,2016年度留学回国人员科技活动项目择优资助经费 （31370704）
