生物技术通报2017，Vol.33Issue(11)：160-165,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0519
氮胁迫对埃氏小球藻生长及油脂积累的影响
Effects of Nitrogen Stress on Growth and Oil Accumulation of Chlorella emersionii
摘要
Abstract
Fast-growing Chlorella emersionii strain SXND-25 with great ability of purifying waste water was used as the target microalgae to investigate the effects of culture condition under varied nitrogen (N) concentration on microalgae biomass and oil yield,aiming at establishing an optimized culture system of such valued chlorella for commercial production of high quality biofuels.BG11 medium was used as the standard medium containing NaNO3 as nitrogen (N) source (1 N).This new strain of C.emersionii was continuously cultured in the medium containing different nitrogen concentrations in the medium set as 0 g/L (0 N),0.375 g/L (1/4 N),0.75 g/L (1/2 N),1.125 g/L (3/4 N),and 1.5 g/L (1 N),respectively.The biomass,oil content and fatty acid composition of the microalgae were analyzed by means of optical density measurement,Nile red staining,direct ester extraction of oil and GC analysis.When cultured in 1 N-medium for 8 d,the C.emersionii increased its biomass up to the maximum level with 3.4 g/L dry weight (DW),whereas oil content was only 28.24％ of DW,with 0.96 g/L oil yield on average.Culture of 8 days at 0 N-medium led to the minimum biomass of the C.emersionii with 0.49 g/L DW,but the highest level of oil accumulation (44.57％ of DW) and small average oil yield (0.22 g/L).Notably,cultivation of 8 d in 1/2 N-medium resulted in the C.emersionii to achieve high biomass (3.2 g/L DW) and moderate oil contents (40.36％ of DW),but the highest oil yield of 1.3 mg/L on average.which was 1.4 folds of oil yield cultured in the standard medium (1 N).Moreover,the fatty acid profile in the Chlorella cultured in 1/2 N-medium for 8 d was detected to be more suitable for production of high-quality biodiesel.In conclusion,comprehensive analysis of various indexes including biomass,oil content and oil yield per unit demonstrates that 0.75 g/L nitrogen concentration can be used as an optimized parameter for developing an effective cultivation system of this special Chlorella strain in producing high-quality biediesel at large scale.关键词
埃氏小球藻(Chlorella emersionii)/缺氮培养/生物量/油脂积累/脂肪酸组成Key words
Chlorella emersionii/nitrogen-deficient cultivation/biomass/oil accumulation/fatty acid components引用本文复制引用
程蔚兰,邵雪梅,宋程飞,史飞飞,季春丽,李润植..氮胁迫对埃氏小球藻生长及油脂积累的影响[J].生物技术通报,2017,33(11):160-165,6.基金项目
国家“948”项目(2014-Z39),山西省煤基重点科技攻关项目(FT-2014-01),山西省重点研发计划重点项目(201603D312005) （2014-Z39）
