生物技术通报2017，Vol.33Issue(11)：194-199,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0388
抗结核海洋放线菌的筛选及菌株HY286生物活性研究
Screening of Anti-Tuberculosis Marine Actinomycetes and the Bioactivity of Strain HY286
摘要
Abstract
Marine actinomycetes are recognized as an important resource of screening new anti-tuberculosis drugs and their precursor compounds.Nine marine actinomycetes strains with efficient antagonism to Mycobacterium smegmatis (Msm) were screened by plate confrontation method from 77 actinomycetes in shallow soil,Zhangzhou.One strain HY286 with the highest inhibitory effect was identified as Actinomadura genus by colony morphological,physiological and biochemical characteristics,and 16S rDNA sequence analysis.The MIC of fermentation crude extract of strain HY286 was 200 μg/mL,indicating that it had a significant inhibitory effect on Msm.The fermentation crude extract of strain HY286 also showed inhibitory effects on Bacillus subtilis and Bacillus pumilus,and the inhibition rate to HeLa and HepG2 was 89.3％ and 94.2％,respectively.关键词
抗结核/马杜拉属/海洋放线菌/筛选/生物活性Key words
anti-tuberculosis/Actinomadura/marine actinomycetes/screening/bioactivity引用本文复制引用
瞿佳,赵玲侠,陈锐,路鹏鹏,孙晓宇,沈卫荣..抗结核海洋放线菌的筛选及菌株HY286生物活性研究[J].生物技术通报,2017,33(11):194-199,6.基金项目
陕西省科技厅项目(2016FWPT-10),陕西省科学院科技计划项目(2014k-35-2) （2016FWPT-10）
