山西医科大学学报2017，Vol.48Issue(12)：1236-1240,5.DOI:10.13753/j.issn.1007-6611.2017.12.008
雷帕霉素对大鼠神经病理性疼痛及脊髓背角神经元凋亡的影响
Effects of rapamycin on neuropathic pain and apoptosis of neurons in spinal cord of rats after spinal nerve ligation
摘要
Abstract
Objective To investigate the effects of rapamycin on neuropathic pain and apoptosis of neurons in the spinal cord of rats after spinal nerve ligation. Methods Thirty male SD rats were randomly divided into three groups(n = 10):sham group,spinal nerve ligation group(SNL group)and rapamycin group(Rap group). After intrathecal catheter implantation between L4 - 5 vertebrae, the rats in SNL group and Rap group underwent L5 spinal nerve ligation. The surgical procedure in sham group was identical except nerve ligation. The rats were injected with rapamycin(0. 1 μg/ 10 μl)per day for 7 d via intrathecal catheter in Rap group after L5 spi-nal nerve ligation,while the rats were injected intrathecally with the same volume of vehicle(5% DMSO)in sham group and SNL group. Paw withdrawal threshold(PWT)and paw withdrawal latency(PWL)were measured at 1,3,5,and 7 d after surgery. At day 7 after surgery,the rats were sacrificed. Ipsilateral L5 spinal cord dorsal horn was examined using transmission electron microscope. Expression of LC3,NeuN and a - caspase3 in the L5 cord dorsal horn were measured by Western blot. Results Compared with sham group,PWT and PWL of rats were significantly decreased at 1,3 day after SNL in SNL group,respectively,and lasted for 7 d(P <0. 05). At day 7 after SNL,PWT[(12. 5 ± 1. 0)g]and PWL[(7. 8 ± 0. 4)s]were decreased by 67% and 44%,respectively(P <0. 05),the levels of LC3 Ⅱ and a - caspase3 expression increased by 96% and 359%,respectively(P < 0. 05),while the levels of NeuN expression decreased by 21% . Compared to SNL group,PWT and PWL of rats were significantly increased in Rap group(P <0. 05). At day 7 after SNL,PWT[(24. 9 ± 1. 6)g]and PWL[(11. 8 ± 0. 8)s]were increased by 99% and 51%,respectively(P <0. 05),the levels of LC3 Ⅱ and NeuN expression increased by 44% and 19%,respectively(P < 0. 05),while the levels of a -caspase3 expression decreased by 27%(P < 0. 05). More autophagosomes were found in Rap group than in SNL group. Conclusion Intrathecal rapamycin may ameliorate SNL-induced neuropathic pain by increasing the autophagy and inhibiting the apoptosis of neu-rons.关键词
雷帕霉素/神经病理性疼痛/自噬/细胞凋亡Key words
rapamycin/neuropathic pain/autophagy/apoptosis分类
医药卫生引用本文复制引用
冯涛,陈功,丁洁,黄凤贞..雷帕霉素对大鼠神经病理性疼痛及脊髓背角神经元凋亡的影响[J].山西医科大学学报,2017,48(12):1236-1240,5.基金项目
广东省深圳市卫生计生系统暨深圳市宝安区科技创新局科研项目( 201507067 ) （ 201507067 ）
