山西医科大学学报2017，Vol.48Issue(12)：1260-1264,5.DOI:10.13753/j.issn.1007-6611.2017.12.013
掺镁透钙磷石对骨质疏松兔骨缺损的成骨效果
Effect of magnesium-doped calcium hydrogenphosphate dehydrate on osteogenesis in osteoporotic bone defect rabbits
摘要
Abstract
Objective To evaluate the effect of 6. 67% magnesium-doped calcium hydrogenphosphate dehydrate on the repair of osteo-porotic radius bone defect in rabbits. Methods Twenty-seven female New Zealand rabbits were used to establish osteoporotic bone defect model,and then randomly divided into 3 groups(n = 9 in each group):control group,calcium hydrogenphosphate dehydrate group and 6. 67% magnesium-doped calcium hydrogenphosphate dehydrate group(6. 67% magnesium-doped group). All rabbits were defected 15 mm long in bilateral radius. The defect was not filled in blank group,while the defect was filled with 6. 67% magnesium-doped calcium hydrogenphosphate dehydrate and calcium hydrogenphosphate dehydrate in the other two groups,respectively. The repair of bone defect was observed by naked eyes,the image changes of bone defect was observed by X-ray,and the expression and distribu-tion of platelet-derived growth factor(PDGF)were observed by immunohistochemistry at 4,8,12 week after operation. Results The repair of bone defect was basically completed at 12 week in 6. 67% magnesium-doped group,but remained concave in the other two groups. The effect in 6. 67% magnesium-doped group was the best among three groups. The immunohistochemistry showed that the ex-pression of PDGF was the highest at 4 week,and then decreased gradually. The expression of PDGF in 6. 67% magnesium-doped group was higher than that of calcium hydrogenphosphate dehydrate group at 4,8 week(P < 0. 05). Conclusion The 6. 67% magne-sium-doped calcium hydrogenphosphate dehydrate can not only promote the healing of bone defect in osteoporosis but also promote the expression of PDGF in the early stage of repair.关键词
桡骨骨缺损/骨质疏松/掺镁透钙磷石/血小板源性生长因子/兔Key words
radial bone defect/osteoporosis/magnesium-doped calcium hydrogenphosphate dehydrate/platelet-derived growth factor/rabbits分类
医药卫生引用本文复制引用
贾婉萍,梁立硕,冯凯悦,周秋娟,梁永强..掺镁透钙磷石对骨质疏松兔骨缺损的成骨效果[J].山西医科大学学报,2017,48(12):1260-1264,5.基金项目
国家自然科学基金资助项目( 81270965 ) （ 81270965 ）
河北省教育厅重点项目( Z D 2015016 ) （ Z D 2015016 ）
