天津中医药大学学报2017，Vol.36Issue(6)：455-460,6.DOI:10.11656/j.issn.1673-9043.2017.06.14
甘草对H2O2诱导大鼠H9c2心肌细胞损伤保护作用的研究
Study on the effects of licorice on H2O2-induced H9c2 myocardial injury
邢蕾蕾 1胡晓慧 1杨瑾 2艾乐 2王怡 1王炎炎1
作者信息
- 1. 天津中医药大学,天津市中药药理学重点实验室,天津 300193
- 2. 天津中新药业集团股份有限公司乐仁堂制药厂,天津 300112
- 折叠
摘要
Abstract
[Objective] To study the effects of licorice on H2O2-induced H9c2 myocardial injury. [Methods] H9c2 cells were treated with H2O2 (100μmol/L for 4 h) to establish a myocardial injury model, and then treated licorice on the concentration of 400 μg/mL and 800 μg/mL for 4 h. The levels of cell viability, lactic dehydrogenas (LDH), superoxide dismutase (SOD), malondialdehyde (MDA), intracellular reactive oxygen species (ROS), intracellular calcium ion concentration , mitochondrial permeability transition pore ( mPTP ) and apoptosis rate were measured . [Results] Licorice can improve cell vitality and activity of SOD in different degree, reduce the LDH, MDA, ROS and Ca2+content, inhibit mPTP opening and apoptosis rate (P<0.05 or P<0.01). [Conclusion] Licorice has the protective effect on H2O2-induced H9c2 myocardial cells.关键词
甘草/H2O2/H9c2/凋亡/氧化应激Key words
licorice/H2O2/H9c2/apoptosis/oxidative stress分类
医药卫生引用本文复制引用
邢蕾蕾,胡晓慧,杨瑾,艾乐,王怡,王炎炎..甘草对H2O2诱导大鼠H9c2心肌细胞损伤保护作用的研究[J].天津中医药大学学报,2017,36(6):455-460,6.
