烟草科技2017，Vol.50Issue(11)：9-15,7.
烟草根黑腐病菌ISSR标记的遗传多样性分析
Genetic diversity analysis of Thielaviopsis basicola by ISSR method
摘要
Abstract
To study the population genetics of Thielaviopsis basicola at molecular level, 54 isolates of T. basicola were collected from Chongqing, Gansu, Guizhou, Henan and Yunnan, and the genetic diversity and population differentiation among geographic populations were analyzed by ISSR method. The results showed that the average of bands amplified by 5 ISSR primers pairs was 9.6(8-12)and the average of polymorphism bands was 3.6(2-5). The Nei's index h, Shannon index I, GSTvalue and the gene flow Nmof total populations were 0.350 7, 0.521 5, 0.338 9 and 0.975 6, respectively. The genetic distances of the 5 populations ranged from 0.113 4 to 0.275 2. The genetic identities between Henan and the other populations were higher with close genetic distances. The genetic identity of Guizhou population with Henan population was higher(0.804 8), with the other populations was lower. NTsys 2.10e cluster analysis indicated that the genetic similarity coefficients of different isolates were from 0.82 to 1.00 and the isolates could be clustered into 5 groups at the genetic similarity coefficient of 0.84. In conclusion, T. basicola maintains rich genetic diversity in China, there exists genetic differentiation among different populations, and the gene flow in populations was large.关键词
烟草根黑腐病菌/简单重复性序列间区(ISSR)/遗传多样性/遗传分化Key words
Thielaviopsis basicola/ISSR/Genetic diversity/Genetic differentiation分类
农业科技引用本文复制引用
窦彦霞,徐林,马冠华,李六英,张世渠..烟草根黑腐病菌ISSR标记的遗传多样性分析[J].烟草科技,2017,50(11):9-15,7.基金项目
中央高校基本科研业务费专项项目"根串珠霉dsRNA病毒多样性研究"(XDJK2015C167) （XDJK2015C167）
重庆市烟草专卖局资助项目"烟草黑胫病菌和赤星病菌致病性分化和病害流行规律研究"(NY20140401070002). （NY20140401070002）
