烟草科技2017，Vol.50Issue(11)：58-65,8.
LC-MS/MS法同时测定烟草中的16种游离氨基酸和6种Amadori化合物
Simultaneous determination of sixteen free amino acids and six Amadori products in tobacco by LC-MS/MS
摘要
Abstract
To develop a rapid method for simultaneous determining 16 free amino acids and 6 Amadori products (APs)in tobacco,a method based on liquid chromatography-tandem mass spectrometry(LC-MS/MS)was developed. After extracting with water,tobacco samples were separated on an Acclaim Explosive E2 column with the gradient elution mobile phase of methanol and 0.1%(V/V)formic acid aqueous solution. The mass spectrometry detection was carried out by means of electrospray ionization in positive mode(ESI +)with multiple reaction monitoring(MRM)method. The results showed that:1)The correlation coefficients(r2)were higher than 0.996 for the all calibration curves,the standard recoveries of the spiked samples ranged from 82.0% to 109.5%,the intra-day and the inter-day relative standard deviations(RSDs)were 0.6%-4.8%(n=6) and 1.3%-10.9%(n=6),respectively. 2)This method was simple,rapid,accurate,and suitable for the simultaneous determination of the free amino acids and APs in tobacco samples.关键词
烟草/氨基酸/Amadori化合物/液相色谱-串联质谱联用(LC-MS/MS)/同时测定Key words
Tobacco/Amino acid/Amadori product/Liquid chromatography-tandem mass spectrometry (LC-MS/MS)/Simultaneous determination分类
轻工纺织引用本文复制引用
张婕,葛永辉,杨慧,朱文静,向章敏,耿召良..LC-MS/MS法同时测定烟草中的16种游离氨基酸和6种Amadori化合物[J].烟草科技,2017,50(11):58-65,8.基金项目
贵州省烟草公司重点科技计划项目"贵州烟叶中性致香物质研究"(2013-07). （2013-07）
