疑难病杂志2017，Vol.16Issue(12)：1189-1192,4.DOI:10.3969/j.issn.1671-6450.2017.12.001
栀子苷对氧化应激所致H9C2心肌细胞损伤的保护作用机制研究
Protective effects ofg eniposide on oxidative stress ind uced injury in H9C2 cells
摘要
Abstract
Objective To explore the protective effect of geniposide on oxidative stress injury and its mechanism in H9C2.Methods The H2O2was used to construct the model of oxidative stress injury in H 9C2 cells.All the H9C2 cells were divided into three groups:PBS group, H2O2group and H2O2+geniposide group, and were stimulated by PBS, H2O2 (200 μmol/L)and H2O2+geniposide(100 μmol/L)according to the different groups.Then cells were cultured in 37℃24h.After that,the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and the level of malon-dialdehyde(MDA)and reactive oxygen species(ROS)were detected.Meanwhile, the mRNA levels of genes of kelch like ECH associated protein 1(KEAP 1)/NF E2 related factor 2(Nrf2)pathways and its downstream gene heme oxygenase 1 (HO-1)and SOD.Cell counting kit 8(CCK-8)and terminal deoxynucleotidyl transferase mediated dUTP biotin nick end la-beling assay(TUNEL)were used respectively to detect the viability and apoptosis of H 9C2.Results The activities of SOD and GSH Px in H9C2 cells were decreased after H2O2,and geniposide could increase the decreased SOD and GSH-Px.Geni-poside also inhibited the production of MDA induced by H 2O2.ROS production was also suppressed by the treatment of geni-poside.In the H2O2treatment group,the mRNA level of KEAP1 was increased and the mRNA level of Nrf 2,HO-1 and SOD-1 were decreased.However,in H2O2plus geniposide group,these pathological changes have been reversed.Meanwhile,the use of geniposide increased cell viability and decreased the apoptosis of H 9C2 cells.Conclusion Geniposide can inhibit the oxidative injury induced by H 2O2via the activation of Keap1/Nrf2 pathway in H9C2 cells.关键词
栀子苷/氧化应激/H9C2心肌细胞Key words
Geniposide/Oxidative stress/H9C2 cells引用本文复制引用
马振国,袁雨培,张鑫,唐其柱..栀子苷对氧化应激所致H9C2心肌细胞损伤的保护作用机制研究[J].疑难病杂志,2017,16(12):1189-1192,4.基金项目
国家自然科学基金重点项目(81530012) （81530012）
中央高校基本科研业务费专项资金(2042017kf0085) （2042017kf0085）
