岩石矿物学杂志2017，Vol.36Issue(6)：894-902,9.
PM2.5降尘诱导A549细胞G2/M期阻滞的机制研究
Mechanism of G2/M phase arrest in A549 cells induced by PM2.5 dust-fall
摘要
Abstract
In this study,A549 cells were exposed to PM2.5 dust-fall of different concentrations.MTT assay was used to evaluate cell viability.Cell morphology was observed through Scanning Electron Microscopy (SEM).Flow cytometry was used to detect the cell cycle induced by PM2.5.Then the expression levels of p53,p21,CDK1,c-myc and lncRNA H19 were detected by RT-PCR,and cyclin B1 was measured by Western-blot.In addition,the expression of p53,c-myc and CDK1 in A549 cell was detected after transferring H19 siRNA,with the purpose of investigating the mechanism of A549 cell cycle arrest induced by PM2.5.The results showed that,compared with the control group,A549 cell viability was declined in a dose-dependent manner and time-dependent effect.It was observed that cell morphology was changed and the cell membrane surface adsorbed a large amount of dust particles.The proliferation of A549 cells was inhibited in G2/M phase after being treated by PM2.5 for 24 h,and the expression of CDK1 and cyclin B1 was decreased by increasing the expression of p53,p21 and H19.In addition,the expression of H19 was successfully inhibited by transferring H19 siRNA,and then the expression of CDK1 was further decreased.It is inferred that exposure to PM2.5 could inhibit the expression of CDK1 and cyclin B1 by activating p53 and p21 activity,induce G2/M phase arrest,and finally inhibit A549 cell proliferation.In addition,after being exposed to PM2.5,lncRNA H19 may play a specific oncogenes role in treated cells in that it participated in the cell cycle progression by binding to p53 and c-myc,and hence low expression of H19 caused G2/M phase arrest more obviously.关键词
PM2.5降尘/A549细胞/G2/M期阻滞/毒性机制Key words
dust-fall (PM2.5)/A549 cell/G2/M phase arrest/cytotoxicity mechanism分类
天文与地球科学引用本文复制引用
杨洁,霍婷婷,王玉琳,董发勤,曾娅莉,邓建军..PM2.5降尘诱导A549细胞G2/M期阻滞的机制研究[J].岩石矿物学杂志,2017,36(6):894-902,9.基金项目
国家自然科学基金项目(41472046,41602033) （41472046,41602033）
四川省科技计划项目(2016JY0045) （2016JY0045）
Projects of the National Natural Science Foundation of China(41472046,41602033) （41472046,41602033）
Projects of the Science and Technology of Sichuan Province,China(2016JY0045) （2016JY0045）
