应用化学2017，Vol.34Issue(12)：1450-1456,7.DOI:10.11944/j.issn.1000-0518.2017.12.170319
嘧啶基双光子荧光染料的设计合成与生物成像
Design and Synthesis of Pyrimidine Based Two-Photon Fluorescence Probe and Its Application in Bioimaging
摘要
Abstract
In the field of biological imaging, two-photon fluorescent dyes with stable optical properties under physiological conditions have broad application prospects.Herein, we designed and synthesized a two-photon fluorescent dye 2-[(1E)-2-[4-(dimethylamino) phenyl] ethenyl]-6-methyl-4(3H)-pyrimidinone(NHP). NHP was obtained by condensation between 2,4-dismethyl-6-pyrimidinol and 4-dimethylaminobenzaldehyde. The chemical structure and optical properties of NHP was characterized by mass spectrometry( MS) , nuclear magnetic resonance spectroscopy ( NMR ) , ultraviolet-visible ( UV-Vis ) absorption spectra and fluorescence emission spectra method as well as the external environment change effect on the emission spectrum.The results show that the absorption peak of NHP is around 400 nm, and the emission peak of which is around 550 nm.The fluorescence emission of NHP is stable in various pH, ions and amino acids conditions. Furthermore, biological experimental results show that NHP is able to image HepG2/SH-SY5Y cells and Drosophila brains with negligible cytotoxicity. All of those indicate that NHP is an ideal two-photon fluorescence dye for bioimaging.关键词
嘧啶衍生物/双光子成像/荧光探针/生物成像Key words
pyrimidine derivatives/two-photon imaging/fluorescent probes/bioimaging分类
化学化工引用本文复制引用
周佳,倪赟,张承武,仇兴汉,赵燕菲,白磊,张高宾,李林..嘧啶基双光子荧光染料的设计合成与生物成像[J].应用化学,2017,34(12):1450-1456,7.基金项目
国家自然科学基金(81672508,61505076)和江苏省高校自然科学研究重大项目(16KJA180004)资助 Supported by the National Natural Science Foundation of China(No.81672508, No.61505076), the Major Projects of Natural Science Research in Colleges and Universities in Jiangsu(No.16KJA180004) （81672508,61505076）
