中风与神经疾病杂志2017，Vol.34Issue(11)：964-968,5.
线粒体钙单向转运体在匹鲁卡品致痫大鼠 海马神经损伤中的作用
Effect of mitochondrial calcium uniporter on hippocampal neuronal damage in pilocarpine-induced seizures
摘要
Abstract
Objective To investigate the effect of mitochondrial calcium uniporter ( MCU) on hippocampal neuronal damage in pilocarpine-induced seizures .Methods 84 male SD rats were randomly divided into 4 groups:control group ,PI-LO group ( divided into 4 subgroups:2 h,8 h,24 h and 72 h) ,Ru360 group and Spermine group .The behavioral changes of each group were observed .Fluorescent staining was used to detect the free calcium concentration of hippocampus mitochon -dria and hippocampal neuronal apoptosis was detected by TUNEL staining .The expressions of Bax , Bcl-2, Cyt C and caspase-3 were detected by Western blotting .Results (1) Compared with control group ,the mitochondrial calcium con-centration and apoptotic neurons in PILO group increased (P<0.05).Compared with control group,expression of Bax, caspase-3 activation and Cyt C release in PILO group increased ,while expression of Bcl-2 decreased (P<0.05).(2)Com-pared with PILO group,Ru360 reduced the mitochondrial calcium concentration and apoptotic neurons (P<0.05).Com-pared with PILO group ,Ru360 reduced expression of Bax ,caspase-3 activation and Cyt C release ,while increased expression of Bcl-2 (P<0.05).(3)Compared with PILO group,Spermine increased the mitochondrial calcium concentration and ap-optotic neurons (P<0.05).Compared with PILO group,Spermine increased expression of Bax ,caspase-3 activation and Cyt C release,while reduced expression of Bcl-2 (P<0.05).Conclusion MCU plays an important role in hippocampal neuronal damage in pilocarpine-induced seizures .Inhibition of MCU can significantly attenuate hippocampal neuronal apop -tosis,and the underlying mechanism maybe through mitochondria-mediated apoptosis pathway .关键词
癫痫/线粒体钙单向转运体/线粒体钙离子/凋亡Key words
Epilepsy/Mitochondrial calcium uniporter/Mitochondrial calcium/Apoptosis分类
医药卫生引用本文复制引用
王亚丽,王翠,余梦嫣,连亚军,谢南昌..线粒体钙单向转运体在匹鲁卡品致痫大鼠 海马神经损伤中的作用[J].中风与神经疾病杂志,2017,34(11):964-968,5.基金项目
国家自然科学基金面上项目(No.81571260) （No.81571260）
