中国病理生理杂志2017，Vol.33Issue(12)：2208-2211,4.DOI:10.3969/j.issn.1000-4718.2017.12.015
miR-221通过下调PTEN促进肺癌A549细胞增殖
miR-221 promotes proliferation of lung cancer A549 cells by down-regu-lating PTEN
郑礼平 1张楠 1梁翠微 1杜均祥 1龚五星1
作者信息
- 1. 珠海市人民医院,暨南大学附属珠海医院肿瘤科,广东珠海519000
- 折叠
摘要
Abstract
AIM:To explore the effect of microRNA-221 (miR-221) on the proliferation of lung cancer A549 cells, and to investigate its mechanism .METHODS: The A549 cells were transfected with miR-221 mimics by Lipo-fectamine 2000.The expression of miR-221 was detected by RT-qPCR.The expression of PTEN at mRNA and protein le-vels was detected by RT-qPCR and Western blot , respectively .The cell proliferation was examined by CCK-8 assay and colony formation assay.The 3'-UTR of PTEN was cloned into luciferase reporter vector and its enzymatic activity was detec-ted to verify whether miR-221 targeted to PTEN.RESULTS:The expression level of miR-221 in the A549 cells was sig-nificantly increased after transfection with miR-221 mimics as compared with negative control group and blank group ( P<0.01).The mRNA and protein levels of PTEN were significantly down-regulated compared with control group and blank group ( P <0.05 ) .In addition , miR-221 over-expression significantly promoted the proliferation of A 549 cells ( P <0.05).Moreover, miR-221 inhibited the enzymatic activity of luciferase reporter vector of PTEN.CONCLUSION:Over-expression of miR-221 significantly promotes the proliferation ability of human lung cancer A 549 cells by down-regulation of PTEN.关键词
肺癌/A549细胞/微小RNA-221/PTEN蛋白/细胞增殖Key words
Lung cancer/A549 cells/MicroRNA-221/PTEN protein/Cell proliferation分类
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郑礼平,张楠,梁翠微,杜均祥,龚五星..miR-221通过下调PTEN促进肺癌A549细胞增殖[J].中国病理生理杂志,2017,33(12):2208-2211,4.
