中国病理生理杂志2017，Vol.33Issue(12)：2252-2258,7.DOI:10.3969/j.issn.1000-4718.2017.12.022
TLR4/NLRP3炎症复合体介导对比剂诱导的
肾小管上皮细胞炎症和损伤
Involvement of TLR4/NLRP3 inflammasome in contrast medium-induced inflammation and injury in renal tubular epithelial cells
林燕 1林佳琼 2谢楚莉 3管小凤 4谭学贤 5黄泽娜6
作者信息
- 1. 广州医科大学附属第三医院 肾内科,广东广州510150
- 2. 南方医科大学基础医学院医学遗传学教研室,广东广州510515
- 3. 中山大学中山医学院生理学教研室,广东广州510080
- 4. 中山大学孙逸仙纪念医院超声科,广东广州510120
- 5. 广州医科大学附属第三医院 病理科,广东广州510150
- 6. 广东省人民医院急危重症医学部,广东省医学科学院,广东广州510080
- 折叠
摘要
Abstract
AIM: To investigate whether Toll-like receptor 4 ( TLR4 ) and Nod-like receptor protein 3 (NLRP3) inflammasome were involved in contrast medium (CM)-induced inflammation and injury in renal tubular epithe-lial cells.METHODS: Iopromide was used to injure NRK-52E cells in the study.The cell viability was measured by CCK-8 assay.The protein levels of TLR4, NLRP3, apoptosis-associated speckle-like protein (ASC), caspase-1 and cleaved caspase-3 were determined by Western blot .The releases of interleukin ( IL )-1βand IL-18 were detected by ELISA .The apoptotic rate was evaluated by Hoechst staining , and mitochondrial membrane potential ( MMP) was analyzed by JC-1 staining.siRNA was transfected into the NRK-52E cells to silence NLRP3 expression.RESULTS:CM decreased the viability of NRK-52E cells (P<0.05).CM also elevated the protein levels of cleaved caspase-3, TLR4, NLRP3, IL-1βand IL-18 (P<0.05).Silencing NLRP3 attenuated CM-induced releases of inflammatory cytokines .Moreover, treat-ment with TLR4 inhibitor TAK-242 or knockdown of NLRP3 by siRNA transfection both attenuated cell apoptosis and loss of MMP caused by CM .CONCLUSION:TLR4/NLRP3 inflammasome takes part in the pathogenesis of CM-induced acute kidney injury , and mediates CM-induced injury and inflammation in renal tubular epithelial cells .关键词
对比剂/肾损伤/炎症/Toll样受体4/Nod样受体蛋白3炎症复合体Key words
Contrast media/Kidney injury/Inflammation/Toll-like receptor 4/Nod-like receptor protein 3 inflammasome分类
医药卫生引用本文复制引用
林燕,林佳琼,谢楚莉,管小凤,谭学贤,黄泽娜..TLR4/NLRP3炎症复合体介导对比剂诱导的
肾小管上皮细胞炎症和损伤[J].中国病理生理杂志,2017,33(12):2252-2258,7.
