中国病理生理杂志2017，Vol.33Issue(12)：2269-2273,5.DOI:10.3969/j.issn.1000-4718.2017.12.025
干扰STAT3基因对哮喘气道损伤的影响
Effect of STAT3 down-regulation on airway injury in asthma
摘要
Abstract
AIM:To investigate the effect of signal transducer and activator of transcription 3 (STAT3) on air-way injury in asthma and its mechanism .METHODS:The expression and activation of STAT 3 in bronchial mucosa tissues of asthmatic patients were measured by Western blot .The expression and activation of STAT 3 in bronchial epithelial cells pretreated with Dermatophagoides pteronyssinus antigen P1 (Derp1) were estimated.Bronchial epithelial cells were trans-fected with STAT3 shRNA.STAT3 expression was measured by Western blot .The cell viability was detected by CCK-8 as-say.The concentrations of TNF-α, IL-1βand IL-6 were detected by ELISA .The content of malondialdehyde ( MDA) and the activity of superoxide dismutase ( SOD) and glutathione peroxidase ( GSH-Px) were also determined for evaluating the status of oxidative stress .The cell apoptosis was analyzed by flow cytometry .RESULTS:The protein level of p-STAT3 was significantly up-regulated in both bronchial mucosa of asthmatic tissues and bronchial epithelial cells pretreated with Derp 1. Knockdown of STAT3 inhibited the releases of TNF-α, IL-1βand IL-6, decreased the content of MDA and enhanced the activity of SOD and GSH-Px with the suppression of cell apoptosis ( P <0.05 ) .CONCLUSION: Down-regulation of STAT3 attenuates Derp1-induced the airway injury .The mechanism may involve that knockdown of STAT3 suppresses the activation of JAK/STAT signaling pathway , the release of asthma-related inflammatory cytokines and oxidative stress in bronchial epithelial cells , thus inhibiting cell apoptosis .关键词
信号转导及转录激活因子3/哮喘/支气管上皮细胞/炎症细胞因子/氧化应激Key words
Signal transducer and activator of transcription 3/Asthma/Bronchial epithelial cells/Inflamma-tory cytokines/Oxidative stress分类
医药卫生引用本文复制引用
杨远舰,张桢,程哲..干扰STAT3基因对哮喘气道损伤的影响[J].中国病理生理杂志,2017,33(12):2269-2273,5.基金项目
河南省卫生厅科技攻关项目(No.201503057) （No.201503057）
河南省高等学校重点科研项目(No.18A320056) （No.18A320056）
河南省卫生科技创新型人才工程(豫卫科2010-52号) （豫卫科2010-52号）
