中国临床药理学杂志2017，Vol.33Issue(19)：1893-1896,4.DOI:10.13699/j.cnki.1001-6821.2017.19.012
缺氧诱导因子-1α与缺氧诱导因子-2α蛋白和mRNA在宫颈癌中的表达及其临床意义
Protein and mRNA expression of hypoxia-inducible factor-1α and hypoxia-inducible factor-2α with cervical cancer and its clinical significance
摘要
Abstract
Objective To evaluate the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in cervical carcinoma.Methods To detect the HIF-1α and HIF-2α expression of 30 cases cervical carcinoma tissue (treatment group) and 30 cases cervical benign tissue (control group) by immunohistochemistry,Western blot and real time polymerase chain reaction (RT-PCR) technology,analysis the correlation of HIF-1 α and HIF-2α expression and clinical pathological.Results Expressions of HIF-1 α mRNA and HIF-2α mRNA in treatment group were (2.06 ± 0.68),(1.62 ± 0.43),had significant difference with control group (all P ＜ 0.01).Immunohistochemistry showed that the positive expression rate of HIF-1α and HIF-2α were 63.33％ and 70.00％ in treatment group,had significant difference with those in control group,which were 16.67％ and 13.33％ (all P ＜ 0.01).Western blot results showed that the HIF-1α and HIF-2α were (0.71 ± 0.01),(0.71 ± 0.03) in treatment group,had significant difference with those in control group,which were (0.53±0.03) and (0.56 ± 0.05) with significant difference (all P ＜ 0.05).HIF-1α was correlated with lymph node metastasis and anemia(all P＜0.05).HIF-2α was correlated with anemia (P ＜0.05).Conclusion The expression levels of HIF-1α,HIF-2α mRNA and protein in significant increase in treatment group,they may play a role of papillary thyroid carcinoma in tumor promotion,and they change in the same direction,suggesting that they may play the same or similar effect in cervical carcinoma through different mechanisms.关键词
缺氧诱导因子-1α/免疫组化/聚合酶链式反应/免疫印迹Key words
hypoxia inducible factor-1α/hypoxia inducible factor-2α/immunohistochemistry/real time polymerase chain reaction/Western blot分类
医药卫生引用本文复制引用
应申鹏,曹学全,刘艳梅,梁勇..缺氧诱导因子-1α与缺氧诱导因子-2α蛋白和mRNA在宫颈癌中的表达及其临床意义[J].中国临床药理学杂志,2017,33(19):1893-1896,4.基金项目
台州市科技计划基金资助项目(1301ky24) （1301ky24）
