中国临床药理学杂志2017，Vol.33Issue(19)：1918-1921,4.DOI:10.13699/j.cnki.1001-6821.2017.19.020
细胞外信号调节蛋白激酶1/2磷酸化及白细胞介素-32在哮喘小鼠气道黏液高分泌中的作用
Effects of phosphorylation of extracellular signal-regulated protein kinase 1/2 and interleukin 32 in airway mucus hypersecretion in asthmatic mice
摘要
Abstract
Objective To explore the effect of U0126 [specific inhibitor of extracellular signal-regulated protein kinase 1/2 (ERK1/2)] and dexamethasone on the protein expression of phosphorylation of ERK1/2 (p-ERK1/2),interleukin 32 (IL-32) and mucins 5 ac (Muc5ac) in lung tissue in asthmatic mice.Methods Forty model mice of bronchial asthma were randomly divided into model group and test A,B,C groups,with 10 mice per group.Another 10 normal mice were selected as control group.Model group was given ovalbumin (OVA) 0.2 mL on the day 1 and 13 by intraperitoneal injection,and the inhalation of 5％ OVA with 30 rain per time for 5 d began on the 19th day.Test A,B,C groups were given 2 mg· kg-1 dexamethasone,30 mg · kg-1 U0126 and dimethylsulfoxide 0.2 mL with intraperitoneal injection,respectively,which was before the treatment of inhalation,and another treatment was the same as model group.Control group was given 0.9％ NaCl,and another treatment was also the same as model group.The expression of IL-32 in bronchoalveolar lavage fluid (BALF) were determined by enzyme-linked immunosorbent assay.The protein expression of Muc5ac,p-ERK1/2 and IL-32 in lung tissue were measured by immunohistochemical method.Results The main indexes in model and test A,B groups were compared:the optical density (OD) ofIL-32 were(0.81 ±0.25),(0.34 ±0.06) and (0.37 ±0.12),the integral optical density (IOD) of Muc5ac were (54462.97 ±6907.17),(7904.62 ± 1639.96) and (7447.74 ± 1233.68),the IOD of p-ERK were (95621.04± 11112.78),(10225.53 ± 1456.72) and (10718.69 ± 2554.13),the IOD of IL-32 were (44870.78 ±7330.11),(15075.80 ± 3490.51) and (16847.16 ± 3485.83).The differences were statistically significant between model group and test A,B groups (all P ＜ 0.01).Conclusion Both p-ERK1/2 and IL-32 were involved the expression and secretion of Muc5ac in asthmatic mice,and they were synergistic.关键词
支气管哮喘/细胞外信号调节蛋白激酶1/2/白细胞介素-32/黏蛋白5acKey words
bronchial asthma/extracellular signal-regulated protein kinase 1/2/interleukin 32/mucins 5 ac分类
医药卫生引用本文复制引用
郑飞彦,张建勇,杨红霞,毕海..细胞外信号调节蛋白激酶1/2磷酸化及白细胞介素-32在哮喘小鼠气道黏液高分泌中的作用[J].中国临床药理学杂志,2017,33(19):1918-1921,4.基金项目
贵州省科技计划基金资助项目(黔科合SY字[2008]3056号01) （黔科合SY字[2008]3056号01）
贵州省科学技术基金资助项目(黔科合J字[2007]2218号) （黔科合J字[2007]2218号）
