中国临床药理学杂志2017，Vol.33Issue(19)：1946-1948,3.DOI:10.13699/j.cnki.1001-6821.2017.19.027
类表皮生长因子域7基因shRNA慢病毒表达载体的构建及鉴定
Construction and identification of lentiviral vector-mediated short hairpin RNA of human EGF-like domain 7 gene
摘要
Abstract
Objective To construct the LV3-human EGF-like domain 7-targeted small hairpin RNA (LV3-hEGFL7-shRNA)-expressing plasmid system.Methods Sequence of hEGFL7-targeted shRNA was designed by Oligo Designer 3.0 based on the mRNA sequence of hEGFL7 which was obtained from the Genbank.They were recombined with the plasmid pGLV3/H1/GFP + Puro,and then those plasmids were identified by gene sequencing to make sure they were correctly connec-ted.Then those plasmids were transfected into 293T cells and fluorescence expression was observed.Results The sequence of EGFL7-shRNA was demonstrated that the inserted sequences were correct,and the titer of virus was 2 × 10s TU · mL-1.Conclusion The hEGFL7-targeted shRNA expressing plasmids were successfully constructed,identification can meet test requirements.关键词
类表皮生长因子域7/RNA干扰/短发夹结构RNA/慢病毒Key words
EGF-like domain 7/RNA interference/short hairpin RNA/lentivirus分类
医药卫生引用本文复制引用
王爱岳,李强,许琼冠,刘达远,徐鹏翔,余丹..类表皮生长因子域7基因shRNA慢病毒表达载体的构建及鉴定[J].中国临床药理学杂志,2017,33(19):1946-1948,3.基金项目
海南省自然科学基金资助项目(814375) （814375）
海南省卫生厅基金资助项目(琼卫2010-31) （琼卫2010-31）
