摘要
Abstract
Objective To explore the protective effect of dexmedetomidine (Dex) pretreatment on the cardiac muscle cell in myocardial ischemia reperfusion of rat.Methods Male rats were divided into 4 groups,7 cases in each group:experimental-L group,experimental-H group (intraperitoneal injection 10,30 μg · kg-1 Dex at the time of 0.5 h before ligatured),model group and sham-operation group (intraperitoneal injection 5 mL · kg-1 0.9％ NaC1 at the time of 0.5 h before ligatured).The myocardial ischemia-reperfusion injury rats model was prepared by ligatured left coronary artery.The myocardial apoptosis was detected by Terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling.The expression of B-cell lymphoma-2 (Bcl-2),Bax in myocardial tissue were detected by immunohistochemical.Results The apoptosis rate (AR,％) in sham-operation group,model group,experimental-L group,experimental-H group were 0.42 ± 0.06,19.73 ±4.12,13.31 ±3.65,10.04 ±2.71 respectively;the Bcl-2 posotive expression index (PEI) in the four groups were 0.25 ± 0.03,5.74 ± 1.06,11.44 ± 2.01,14.71 ± 2.13 respectively;the Bax PEI in the four groups were 0.27 ±0.04,19.95 ± 5.01,15.02 ± 4.82,12.13 ± 3.14 respectively;the Bcl-2/Bax in the four groups were 0.93 ± 0.05,0.29 ± 0.04,0.76 ± 0.06,1.20 ± 0.07 respectively.Compared with sham-operation group,the AR,Bcl-2 PEI,Bax PEI in model group increased significantly (P ＜0.01),Bcl-2/Bax decreased significantly(P ＜ 0.01).Compared with model group,the Bcl-2 PEI and Bcl-2/Bax in the four groups in experimental-L group,experimental-H group increased significantly (P＜0.01),the AR of myocardial cell and Bax PEI in the four groups decreased significantly (P ＜ 0.01).Conclusion Dex can inhibit the myocardial cell apoptosis of myocardial ischemia reperfusion rat through the up-regulation of Bcl-2 protein expression and the down-regulation of Bax protein expression.关键词
缺血再灌注/右美托咪定/心肌细胞/凋亡Key words
ischemia reperfusion/dexmedetomidine/cardiac muscle cell/apoptosis分类
医药卫生
