中国临床药理学杂志2017，Vol.33Issue(20)：2039-2042,4.DOI:10.13699/j.cnki.1001-6821.2017.20.013
过表达α2,3-唾液酸转移酶基因对乳腺癌MDA-MB-231细胞侵袭能力的影响
Effects of overexpression of α 2,3-sialyltransferase I on invasion of breast cancer MDA-MB-231 cells
摘要
Abstract
Objective To investigate the effect of overexpression of α 2,3-sialyltransferase (ST3Gal Ⅲ) on abilities of invasion in breast cancer cells MDA-MB-231.Methods ST3Gal Ⅲ gene lentiviral expression vector was transfected into MDA-MB-231 cells.Transfected cells were divided into 3 groups:blank group (Mock cells,M),control group (Parent cells P),experimental group (ST3Gal Ⅲ ST3).The expression of ST3Gal Ⅲ mRNA and protein were detected by Quantitative Real-time PCR(RT-PCR) and Western blot.The content of α2,3-sialic acids was analyzed by flow cytometry.The ability of migration and invasion in MDA-MB-231 cells were detected by cell adhesion assay and Transwell assay,respectively.Results The expression levels of ST3Gal Ⅲ mRNA and protein examined by RT-PCR and Western blot in control group and experimental group were 0.91 ± 0.04 and 1.85 ± 0.08 respectively.The expression levels in experimental group were 1.84-fold and 1.76-fold higher than control group(P ＜ 0.05).The amount of SLeX on the cell surface in experimental group was 92.86 ± 4.41 and was 1.61-fold higher than control group,which were significantly increased compared with that in the blank group and control group (P ＜ 0.05).OD570nm values of the adhesion assay in the in the blank group,the control group and the experimental group were 0.32 ± 0.02,0.29 ± 0.01 and 0.46 ±0.03,respectively,while those of the invasion assay were 0.93 ±0.02,0.81 ±0.01 and 1.46 ±0.13,and there was significant difference between the experimental group and the control group (P ＜ 0.05).Conclusion Overexpression of ST3Gal Ⅲ gene could enhance the invasion ability of MDA-MB-231 cells.关键词
过表达/α2,3-唾液酸转移酶/转染/侵袭Key words
overexpression/α 2,3-sialyltransferase/transfection/invasion分类
天文与地球科学引用本文复制引用
梁文举,王勤,刘莹,孙尚国,崔红霞..过表达α2,3-唾液酸转移酶基因对乳腺癌MDA-MB-231细胞侵袭能力的影响[J].中国临床药理学杂志,2017,33(20):2039-2042,4.基金项目
国家自然科学基金青年科学基金资助项目(81302308) （81302308）
黑龙江省自然科学基金资助项目(H201353) （H201353）
黑龙江省大学生创新创业训练计划基金资助项目 （）
