摘要
Abstract
Objective To investigate the effect of sevoflurane maternal anesthesia on cognitive function in offspring rats,and to explore the mechanism.Methods A total of 27 SD female rats aged 7 weeks were randomly divided into test group-1 (n =9),test group-2 (n =9) and control group (n =9).At 5-7 d after pregnancy,test group-1,test group-2 were inhaled 1.8％ sevoflurane for 4,8 h,control group only inhaled oxygen for 8 h,oxygen flow rate 2 L · min-1 Each group was randomly selected 6 offspring rats,and the morris water maze system was used to determine its cognitive function on the 30 d after birth,the expression of NR2B mRNA and HGN mRNA in hippocampus were detected by reverse transcription polymerase chain reaction (RT-PCR),the expression of NR2B protein in hippocampus was detected by immunohistochemistry.Results Morris water maze system showed that there was no significant difference in three groups in the escape latency,the number of crossing the platform and the time of residence in the platform quadrant.The NR2B mRNA and HGN mRNA in test group-1 were 0.79 ± 0.11,0.66 ± 0.08.The NR2B mRNA and HGN mRNA in test group-2 were 0.77 ± 0.09,0.65 ± 0.07.The NR2B mRNA and HGN mRNA in control group were 0.78 ± 0.08,0.68 ± 0.07,with no significant difference in three groups (P ＞ 0.05).The average gray values of NR2B protein positive cells in test group-1,test group-2 and control group were 99.68 ± 17.68,97.62 ± 18.63,101.38 ± 15.52.The average gray values of HGN protein positive cells in test group-1,test group-2 and control group were 86.49 ± 22.04,89.14 ±20.15,92.15 ± 22.51,with no significant difference in three groups (P ＞ 0.05).Conclusion Early pregnancy sevoflurane maternal anesthesia has no affect in the expression of NR2B mRNA and HGN mRNA and NR2B protein in hippocampus of offspring SD rats,whether it is related to the dose of sevoflurane anesthesia need further study.关键词
七氟烷/母体麻醉/孕早期/认知功能/机制Key words
sevoflurane/maternal anesthesia/early pregnancy/cognitive function/mechanism分类
医药卫生
