中国临床药理学杂志2017，Vol.33Issue(20)：2065-2067,3.DOI:10.13699/j.cnki.1001-6821.2017.20.020
高效液相色谱-质谱联用法测定人血浆中比卡鲁胺的浓度
Determination of bicalutamide concentration in human plasma by HPLC-MS/MS
毕国放 1张应福 1张媛媛 1张丽君 1王晓芳 1李媛媛 1程龙妹 1曲恒燕1
作者信息
- 1. 军事医学科学院附属医院临床药理学研究室,北京100071
- 折叠
摘要
Abstract
Objective To determine the bicalutamide concentration in human plasma by established HPLC-MS/MS method.Methods The plasma was treated with protein precipitation,Bicalutamide-d4 was used as internal standard,chromatographic column:Agilent ZORBAX SB-C18 column (2.1 mm ×S0.0 mm,5 μm),column temperature:20 ℃,mobile phase:methanol-0.1％ formic acid and 2 mmol · L-1 ammonium formate,isocratic elution,flow rate:0.35 mL · min-1,mass concentration of the sample was measured with negative ion scan and multiple reaction monitoring mode.The specificity,lower limit of quantitation and standard curve,precision and recovery rate and stability as well as the matrix effect were investigated.Results The standard curve of bicalutamide was y =6.82 × 10-3x + 1.33 × 10-2 (r =0.999 1),and has good linear relationship in 10-1500 ng · mL-1,the lowest concentration was 10 ng · mL-1.Intra-day and inter-day RSD of blood samples were less than 15％,the average recovery was ＞ 95％,and stability was good.Conclusion The method was simple,rapid,sensitive and accurate,specific,suitable for the determination of bicalutamide concentration in human plasma.关键词
比卡鲁胺/液相色谱-质谱联用法/血药浓度Key words
bicalutamide/HPLC-MS/MS/plasma concentration分类
医药卫生引用本文复制引用
毕国放,张应福,张媛媛,张丽君,王晓芳,李媛媛,程龙妹,曲恒燕..高效液相色谱-质谱联用法测定人血浆中比卡鲁胺的浓度[J].中国临床药理学杂志,2017,33(20):2065-2067,3.
