中国临床药理学杂志2017，Vol.33Issue(20)：2068-2072,5.DOI:10.13699/j.cnki.1001-6821.2017.20.021
LC-MS/MS与2D-LC/UV法测定奥氮平血药浓度的比较
Comparison the methods of LC-MS/MS and 2D-LC/UV for determination of olanzapine in human serum
摘要
Abstract
Objective The LC-MS/MS method and 2D-LC/UV method were used to compared the consistency and correlation of olanzapine serum concentration.Methods Quality control samples and 52 serum samples of olanzapine were detected by LC-MS/MS and 2D-LC/UV method respectively.The consistency of results was compared by Bland-Altman method,and the correlation was analyzed by Pearson correlation analysis and Passing-Bablok regression.Results According to the Bland-Altman method,the percentage of the limit can be obtained:96.15％ LoA =(-1.53,3.81).And 96.15％ difference value was within the limit and the consistency of the two methods was good.According to the Pearson linear regression analysis,Passing-Bablok regression equation was OLZLC-MS/MS =1.02 × OLZ2D-LC/UV-1.18 and correlation coefficient was 0.999 (P ＜ 0.01) with good correlation.Compared to the middle and high serum concentration,the relative deviation testes by two methods in low serum concentration(＜ 15 ng · mL-1) was larger.Conclusion The 2D-LC/ UV method and LC-MS/MS method have the good consistency and correlation in detecting olanzapine serum concentration,which can be used for conventional therapeutic drug monitoring(TDM).关键词
全自动二维液相色谱法/高效液相串联质谱法/奥氮平/血药浓度/一致性Key words
2D-LC/UV/LC-MS/MS/olanzapine/serum concentration/consistency分类
医药卫生引用本文复制引用
陈雨晴,周艳萍,滕婧,王峰,温预关..LC-MS/MS与2D-LC/UV法测定奥氮平血药浓度的比较[J].中国临床药理学杂志,2017,33(20):2068-2072,5.基金项目
广东省自然科学研究基金资助项目(2015A030313808) （2015A030313808）
