中国临床药理学杂志2017，Vol.33Issue(20)：2073-2076,4.DOI:10.13699/j.cnki.1001-6821.2017.20.022
HPLC法测定龙胆健肝胶囊中5种有效成分的含量
Simultaneous determination of five components in the Long-Dan-Jian-Gan capsules by HPLC
摘要
Abstract
Objective To develop a method for determining the contents of 5 main active compounds in Long-Dan-Jian-Gan capsules.Methods Chromatographic separation was carried out on Agilent ZORBAX SB-C18(4.6 mm ×250 mm,5 μm).The mobile phase was methanol and phosphoric acid in aqueous solution (0.1％) with gradient elution.The flow rate of mobile phase was maintained at 1.0 mL · min-1 The detection wavelength was set at 280 nm.Investigation of specificity,calibration curves,lower limit of quantitation,precision,recovery and stability were investigated.Results Gentiopicrin,polydatin,scoparone,hesperidin and emodin had good linerities with concentrations in the ranges of 9.9-158.1,4.2-66.8,10.5-167.8,0.8-13.1,2.7-42.5 ng· mL-1,respectively.The lower limit of quantitation were 98.80,41.76,104.88,81.60,265.60 ng · mL-1,respectively.The average recoveries of high,medium and low concentrations were 94.19％-99.54％,94.90％-101.07％,94.45％-102.04％,RSD ≤2.32％.The precision of the instrument was good.Conclusion This method of the operation was simple,quick,accurate,and can be used for quality control of Long-Dan-Jian-Gan capsules.关键词
龙胆健肝胶囊/高效液相色谱法/有效成分/含量测定Key words
Long-Dan-Jian-Gan capsule/HPLC/active ingredient/content determination分类
医药卫生引用本文复制引用
郑旭光,颜文,张振杰,段立广,张磊,李德强,杨树民..HPLC法测定龙胆健肝胶囊中5种有效成分的含量[J].中国临床药理学杂志,2017,33(20):2073-2076,4.基金项目
河北省食品药品监督管理局科技基金资助项目(PT2014058) （PT2014058）
