中国免疫学杂志2017，Vol.33Issue(12)：1761-1764,4.DOI:10.3969/j.issn.1000-484X.2017.12.001
Lactobacillus casei Zhang对扑热息痛诱导的小鼠急性肝损伤的保护作用
Protective effect of Lactobacillus casei Zhang against Acetaminophen-induced acute liver injury in mice
摘要
Abstract
Objective:To investigate the protective effects of Lactobacillus casei Zhang ( Lcz) on mice with Acetaminophen (APAP)-induced acute liver injury.Methods:Mice were randomly divided into five groups:Control group (ctrl),APAP-induced liver injury group ( APAP ) , N-Acetylcysteine positive control group ( NAC ) , Lcz preventive group ( Lcz/APAP ) and Lcz control group (Lcz).Mice were orally administrated with Lcz (1×109 CFU/ml) for 30 continuous days prior to APAP exposure.Positive control group were intraperitoneally injected with 150 mg/kg NAC 1 h before APAP challenge.Mice in the APAP,NAC and Lcz/APAP group were intraperitoneally injected with 300 mg/kg APAP.Blood and liver samples were collected 18 h post APAP challenge.The expression of HO-1,SOD2,Bcl-2 and TLR4 were detected by Western blot.Results: Lcz could significantly inhibit APAP-induced increase in ALT and AST levels.Furthermore,pretreatment with Lcz increased the hepatic expression of HO-1,SOD2 and Bcl-2,while suppressing the expression of Toll-like receptor 4 (TLR4).Conclusion:Lcz can protect against APAP-induced acute liver injury in mice.The hepato-protective effects of Lcz are associated with its anti-oxidative and anti-inflammatory capacity.关键词
LactobacilluscaseiZhang/扑热息痛/超氧化物歧化酶2/血红素氧化酶/Bcl-2Key words
Lactobacillus casei Zhang/Acetaminophen/SOD2/HO-1/Bcl-2分类
医药卫生引用本文复制引用
张晓慧,颜妍,赵世敏,王昕旭,刘春妍,谢基明,王玉珍,武春燕..Lactobacillus casei Zhang对扑热息痛诱导的小鼠急性肝损伤的保护作用[J].中国免疫学杂志,2017,33(12):1761-1764,4.基金项目
本文为国家自然科学基金(81260662,81560677)、内蒙古杰出青年培育基金( 2016JQ08 )和内蒙古自然科学基金( 2016MS0836, 2015MS0884). （81260662,81560677）
