中国农业科技导报2017，Vol.19Issue(12)：14-23,10.DOI:10.13304/j.nykjdb.2017.0224
iTRAQ技术及其在水稻蛋白质组学中的应用研究进展
Research Advance in iTRAQ Technology and Its Application in Proteomics of Rice
摘要
Abstract
Isobaric tags for relative and absolute quantitation (iTRAQ) is a novel high-throughput technique for protein sequencing.This technology combined with high-resolution mass spectrometer is a powerful means for carrying out qualitative and quantitative analysis up to 8 samples simultaneously.This method can test wide range of protein with low limitation,and yet has reliabal and accurate results.Recently,iTRAQ technology has been widely used in studying plant structure,function and expression,etc..Rice (Oryza sativa L.) is a main grain crop for human,and also the first cereal model plant completed genome sequencing.It is of great significance to study rice growth,development and regulation mechanism at the proteome level.This paper summarized the principle,operation procedure,advantages and disadvantages of iTRAQ technology,and focused on analyzing rice proteomics research in plant tissue and organ,subcellular level,environment stress,hormone induce,and rice mutant,etc..Finally this paper prospected the application of iTRAQ technology in rice proteomics.关键词
同位素标记相对和绝对定量技术(iTRAQ)/水稻/蛋白质组学/胁迫/激素/突变体Key words
isobaric tags for relative and absolute quantitation (iTRAQ)/rice/proteomics/stress/hormone/mutant分类
生物科学引用本文复制引用
陈凌华,程祖锌,许明,郑金贵..iTRAQ技术及其在水稻蛋白质组学中的应用研究进展[J].中国农业科技导报,2017,19(12):14-23,10.基金项目
福建省教育厅中青年教师教育科研项目(JAT160685)资助. （JAT160685）
