中国农业科学2017，Vol.50Issue(20)：4003-4011,9.DOI:10.3864/j.issn.0578-1752.2017.20.014
采后ABA处理促进'陇薯3号'马铃薯块茎愈伤形成
Postharvest ABA Treatment Promote Wound Healing of Potato'Longshu No.3' Tubers
摘要
Abstract
[Objective]The experiment was conducted to study the effects of exogenous abscisic acid (ABA) treatment on wound healing of the potato tubers 'Longshu No.3', to analyze the role of phenylpropane metabolism and antioxidant enzymes in wound healing. [Method]The potato cultivar 'Longshu No.3' was used as materials. The tubers were artificially damaged, then treated with different concentrations ABA, and healed under dark condition ((20±3)(℃, RH (80±5)% )).ted tubers was evaluated. The changes of activity of phenylpropane metabolism key enzymes, peroxidase and polyphenol oxidase were determined. The content of metabolites was determined at wounded site of tubers treated with 100 mg?L-1ABA during healing. The formation of healing tissue was observed by scanning electron microscopy. [Result]ABA treatment at different concentrations effectively promoted wound healing of tubers, the screened optimum concentration was 100 mg?L-1. ABA treatment at 100 mg?L-1 significantly increased the activity of phenylalnine ammonialyase, cinnamic acid hydroxylase, 4-coumaroyl-coenzyme A-ligase and cinnamyl alcohol dehydrogenase, sped the accumulation of total phenols, flavonoids and lignin at wounded site of tubers. In which, the activity of phenylalanine ammonialyase showed bimodal change, the treatment was 253.07% and 125.18% higher than the control after 3 days and 9 days of healing. The content of total phenols and flavonoids of the treatment showed 48.60% and 50.42%higher than that of the control. Moreover, the activities of peroxidase and polyphenol oxidase were significantly enhanced at wounded site of tubers. Observation of scanning electron microscopy showed that the formation of closing layer and wound periderm of tubers were accelerated after ABA treatment. [Conclusion]Exogenous ABA treatment accelerates the formation of wound periderm of potato tubers by promoting the phenylpropane metabolism, and increasing the activities of peroxidase and polyphenol oxidase.关键词
马铃薯块茎/愈伤/ABA/苯丙烷代谢/抗氧化酶Key words
potato tubers/wound healing/abscisic acid/phenylpropane metabolism/antioxidant enzyme引用本文复制引用
李雪,吴觉天,王毅,姜红,毕阳,司敏,张静荣,徐洁..采后ABA处理促进'陇薯3号'马铃薯块茎愈伤形成[J].中国农业科学,2017,50(20):4003-4011,9.基金项目
国家自然科学基金(3157102083)、国家自然科学基金(地区科学基金)(31460412)、马铃薯产业体系(CARS-10-P18) （3157102083）
