中国比较医学杂志2017，Vol.27Issue(12)：46-50,114,6.DOI:10.3969.j.issn.1671-7856.2017.12.008
谷甾醇-3-O-葡萄糖苷对瘢痕疙瘩成纤维细胞增殖、凋亡及胶原合成的影响
Effect of sitostero-3-O-glucoside on proliferation, apoptosis and collagen synthesis of keloid fibroblasts
摘要
Abstract
Objective To explore the effect of sitostero-3-O-glucoside on proliferation, apoptosis and collagen synthesis of keloid fibroblasts ( KF) . Methods Cell viability was measured by MTT assay after cells were cultured with 0 (control), 3. 125, 6. 25, 12. 5, 25, 50, 100 μg/mL sitostero-3-O-glucoside. Cell proliferation was measured by EdU staining. Cell apoptotic rate and cell cycle were measured by flow cytometry. The level of COL I and COL III was detected by enzyme linked immunosorbent assay (ELISA). Results 3. 125, 6. 25, 12. 5, 25, 50, 100 μg/mL sitostero-3-O-glucoside reduced cell viability ( P< 0. 01 ) , increased inhibitory rate of cell proliferation ( P< 0. 01 ) , IC50 =27. 54 ± 2. 18 μg/mL. Compared with the control group, 12. 5, 25, 50μg/mL sitostero-3-O-glucoside reduced the cell proliferation rate (P< 0. 01), increased the cell early and late apoptotic rates (P< 0. 01), the cell cycle was arrested at G1 phase (P< 0. 01), and the levels of COL I and COL III were down-regulated (P< 0. 01). Conclusions The results show that sitostero-3-O-glucoside can significantly inhibit the proliferation, induce cell apoptosis and inhibit the synthesis of collagen of keloid fibroblasts.关键词
谷甾醇-3-O-葡萄糖苷/瘢痕疙瘩成纤维细胞/增殖/凋亡/抑制Key words
Sitostero-3-O-glucoside/Keloid fibroblasts ( KF )/Collagen fibers/Proliferation/Apoptosis/Inhibition分类
医药卫生引用本文复制引用
吕经纬,王佳婧,胡刚..谷甾醇-3-O-葡萄糖苷对瘢痕疙瘩成纤维细胞增殖、凋亡及胶原合成的影响[J].中国比较医学杂志,2017,27(12):46-50,114,6.基金项目
吉林省教育厅"十二五"科学技术研究规划项目(吉教科合字[2014]第451号). （吉教科合字[2014]第451号）
