中国兽医科学2017，Vol.47Issue(11)：1341-1348,8.DOI:10.16656/j.issn.1673-4696.2017.11.001
猴麻疹病毒抗体免疫梳检测方法的建立及应用
Establishment and application of a new immune-comb assay for the detection of antibodies against simian measles virus
摘要
Abstract
To research a rapid assay for the detection of antibodies against simian measles virus (MeV),in this experiment the immuno combarray(IC) was developed using MeV recombinant MeV-32 protein as antigen diagnostic reagents.In result,the optimum coating amount of the antigen was 0.1 mg/mL.The prepared ICwas able to specifically detect the positive sera against MeV,and there were no cross reactions with the other viruses.The MeV-32 protein was able to sensitively detect 1∶ 100 times MeV positive serum dilution.The same sample was repeated 3 times,and the coefficient of variation(CV) was less than 10％.In the detection of 10 suspected monkey serum samples using the immune detection method,a consistent rate of the comb with the ELISA test was 100％,and Kappa was 1.000.Theabove-mentioned results showed that the developed immuno combarray had high sensitivity,strong specificity,good repeatability and practicability.关键词
猴麻疹病毒/MeV-32蛋白/免疫梳/快速检测方法/抗体Key words
simian measles virus/MeV-32 protein/IC/rapid assay/IgG分类
农业科技引用本文复制引用
李丹丹,张体银,杨俊兴,邱索平,刘忠梅,高慎阳..猴麻疹病毒抗体免疫梳检测方法的建立及应用[J].中国兽医科学,2017,47(11):1341-1348,8.基金项目
国家质检总局科技项目(2013IK031、2015IK089) （2013IK031、2015IK089）
福建省科技计划重点项目(2014N0001) （2014N0001）
海南省应用技术研究与开发专项项目(ZDXM20130025) （ZDXM20130025）
广东检验检疫局科技计划项目(2011GDK44、2013GDK04) （2011GDK44、2013GDK04）
