中国兽医科学2017，Vol.47Issue(11)：1357-1362,6.DOI:10.16656/j.issn.1673-4696.2017.11.003
猪非典型瘟病毒的基因组特征分析及荧光定量RT-PCR检测方法的建立
Genomic characterization and development of real-time RT-PCR for the detection of atypical porcine pestivirus
许古明 1葛士坤 1张凯照 1刘健新 1任旭皎 1崔灏 1罗畅 1宁章勇1
作者信息
- 1. 华南农业大学兽医学院,广东广州 510642
- 折叠
摘要
Abstract
The most recent researches showed that atypical porcine pestivirus(APPV) was prevalent in swine herds in China and caused serious losses.In this research,thewhole genome of APPV GD3 strain was amplified by RT-PCR,and the genome sequence and its partial coding genes were genetically analyzed.According to the structural protein E2 gene sequence encoded by APPV genome,a real-time RT-PCR method was established for detection of APPV.The whole genome sequence of APPV shared from 83.1％to 83.4％ homology with German,American,Dutch and Spanish strains and APPV located at a different branch.The homologies between GD3 and GD1,GD2 and GD strains were 99.5％,99.6％ and 83.4％,respectively.The homology of E2,Npro and Erns encoded by GD3 strains of APPV with currently available strains were from 83.1％to 99.7％,80.2％to 99.8％and 81.9％to 99.5％,respectively.The real-time RT-PCR method established inthisstudywashighly specific,simple,time-saving and the detection limit was 10 copies /μL.It will be well used in veterinary clinical detection.关键词
猪非典型瘟病毒/序列分析/荧光定量RT-PCRKey words
atypical porcine pestivirus/sequence analysis/real-time RT-PCR分类
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许古明,葛士坤,张凯照,刘健新,任旭皎,崔灏,罗畅,宁章勇..猪非典型瘟病毒的基因组特征分析及荧光定量RT-PCR检测方法的建立[J].中国兽医科学,2017,47(11):1357-1362,6.
