中国兽医科学2017，Vol.47Issue(11)：1363-1370,8.DOI:10.16656/j.issn.1673-4696.2017.11.004
赛鸽新城疫病毒南京分离株的生物学特性及其F和HN基因的分析
Biologic characterization and F and HN gene analysis of racing pigeon Newcastle disease virus strain from Nanjing
摘要
Abstract
One strain of pigeon Newcastle disease virus(NDV) was isolated from a diseased racing pigeon loft suspected to be infected with NDV in Nanjing.This NDV isolate was named Pi/NJ/CH/4416/2016 (ND4416) and purified by using specific pathogen-free(SPF) eggs.Biological tests showed that this isolate showed high pathogenicity to pigeons,but lower to chickens.The resulting RNA samples were converted to cDNAs,followed by PCR amplification and sequencing of F and HNgenes.The cleavage site of the fusion protein(F) was 112RRQKRF117,a feature generally associated with virulent NDV strains.Phylogenetic analysis based on F and HNgene sequences showed that this strain belonged to Class Ⅱ,Ⅵb subgenotype.In addition,Pi/NJ/CH/4416/2016 and other strains prevailing in China were more closely related to the PPMV-1/Belgium/11-09620/2011(JX901124.1) strain.It supported that epidemic pigeon NDV isolates in China might originate from Belgium.Pi/NJ/CH/4416/2016 had a far genetic relationship from the classic vaccine strain La Sota (AF077761.1).Bioinformatics analysis revealed that a linear epitope tope consisting of amino acids 491-500 of HNprotein of this pigeon strain was different from La Sota.In addition,hemagglutination inhibition test revealed a noticeable antigenic difference between them.关键词
赛鸽/鸽新城疫/生物学特性/基因型Ⅵ亚型/F基因/HN基因Key words
racing pigeon/pigeon Newcastle disease/biological characteristics/genotype Ⅵb/F gene/HN gene分类
农业科技引用本文复制引用
熊晓妍,孙兴臣,李敏婕,耿文学,万玉萌,孔志龙,陈闻,许家荣..赛鸽新城疫病毒南京分离株的生物学特性及其F和HN基因的分析[J].中国兽医科学,2017,47(11):1363-1370,8.基金项目
句容市科委项目(NY2013029) （NY2013029）
镇江市农业科技支撑项目(SBK2014022021) （SBK2014022021）
南京农业大学教改项目(2013Y021) （2013Y021）
南京农业大学动物医学院教育教学改革研究项目 （）
赛鸽疾病诊断的研究与防治项目 （）
