中国兽医科学2017，Vol.47Issue(11)：1371-1377,7.DOI:10.16656/j.issn.1673-4696.2017.11.005
DF-1细胞中泛素-蛋白酶体系统对新城疫病毒复制的影响
Influence of the ubiquitin-proteasome system on NDV replication in DF-1 cells
摘要
Abstract
In order to investigate whether the ubiquitin-proteasome system(UPS)of DF-1 cells was involved in the replication of Newcastle disease virus(NDV)in DF1 cells,Herts/33 strain was used to infect DF-1 cells,and the cell samples were subjected to Western-blot experiments and the three proteolytic activity changes of the 26S proteasomes were detected.At the same time,four kinds of ubiquitinproteasome inhibitors were used after the cells were infected with NDV and the virus TCID50 values were determined.The effects of inhibitors treatment and DMSO treatment on NDV replication were compared.As well as the growth curve of NDV with the treatment of MG-132 or DMSO.The results showed that the levels of ubiquitinated proteins in DF-1 cells did not change significantly after NDV infection,but the proteolytic activity of 26S proteasomes was significantly increased.After using ubiquitin-proteasome inhibitors the proliferation of NDV was significantly inhibited,suggesting that the ubiquitin-proteasome system of DF-1 cells was involved in the replication of NDV in DF-1 cells.关键词
新城疫病毒/泛素-蛋白酶体系统/抑制剂/复制Key words
Newcastle disease virus/ubiquitin-proteasomes system/inhibitors/ubiquitin activating enzyme El inhibitor/replication分类
农业科技引用本文复制引用
曲昱蓉,詹媛,仇旭升,谭磊,孙英杰,宋翠萍,孟春,丁铲..DF-1细胞中泛素-蛋白酶体系统对新城疫病毒复制的影响[J].中国兽医科学,2017,47(11):1371-1377,7.基金项目
国家自然科学基金项目(31372421,31530074) （31372421,31530074）
公益性行业(农业)科研专项(201303033) （农业）
