中国兽医科学2017，Vol.47Issue(11)：1406-1410,5.DOI:10.16656/j.issn.1673-4696.2017.11.010
绵羊BST-2B基因的原核表达及多克隆抗体的制备
Prokaryotic expression and polyclonal antibody preparation of BST-2B gene in ovis aries
摘要
Abstract
In order to express recombinant protein of BST-2B gene from 0vis aries,oBST-2B gene was amplified from sheep lung cells by RT-PCR and cloned the fragment into pGEX-4T-1 vector to construct pGEX-4T-oBST/2B recombinant expression plasmid.The recombinant plasmid was transformed into E.coli BL21 (DE3)and induced with IPTG.The recombinant protein was purified and used to immunize BALB/c mice to obtain a polyclonal antibodies against oBST-2B.Western-blot analysis and indirect immunofluorescence assay showed that the polyclonal anti-bodies had a specifical immunogenicity.The oBST-2B protein and polyclonal antibodies may provide a basal bio-material for researches of sheep diseases and the biofunctions of oBST-2B protein.关键词
BST-2B基因/原核表达/多克隆抗体Key words
BST-2B gene/prokaryotic expression/polyclonal antibody分类
农业科技引用本文复制引用
刘腾,张莉,谭永贵,郭慧敏,朱杰,缪秋红,李传峰,陈宗艳,刘光清..绵羊BST-2B基因的原核表达及多克隆抗体的制备[J].中国兽医科学,2017,47(11):1406-1410,5.基金项目
国家重点研发计划项目(2016YFD0500108) （2016YFD0500108）
国家自然科学基金项目(31502068) （31502068）
上海市科技兴农重点攻关项目(2016043) （2016043）
公益性农业科研专项(201303046) （201303046）
中央级公益性科研院所基本科研业务费专项(2016JB01) （2016JB01）
