中国兽医科学2017，Vol.47Issue(11)：1461-1467,7.DOI:10.16656/j.issn.1673-4696.2017.11.018
Nesfatin-1/NUCB2在犬下丘脑-垂体-卵巢轴相关组织上表达与分布的研究
Study on the expression and distribution of nesfatin-1/NUCB in hypothalamus-pituitary-ovary axis relevant tissues of canines
摘要
Abstract
This experiment aimed to explore the location of nesfatin-1 protein and NUCB2 in the reproductive tissues of female dogs.Samples were obtained from puppy(1.5M),prepuberty(4M),puberty (7.5M) and adulthood (24M) female dogs,respectively.Nesfatin-1 protein distribution was determined using immunohistochemistry,andNUCB2mRNA expression in different developmental female dog reproductive axis was measured by real-time quantitative polymerase chain reaction,In result,nesfatin-1 protein was detected in the lateral hypothalamic area,supraoptic nucleus,pituitary gland,oocytes and interstitial substance of ovaries.Additionally,in pituitary and ovary,NUCB2 mRNA expression showed a significant upward trend from puppy to puberty stage(P＜0.01),and swiftly decreased at adulthood (P＜0.01).In the hypothalamus,there was a significant and continuing increase trend from puppy to adulthood stage(P＜0.01).In conclusion,the nesfatin-1 distributed in the reproductive axis tissues widely.TheNUCB2mRNA level peaked at a puberty stage,indicating that nesfatin-1 may regulate the initial of puberty.The NUCB2 mRNA level in ovary was higher than that in the other reproductive organs,suggesting that nesfatin-i may participate in the development and function of the ovaries.关键词
Nesfatin-1/NUCB2 mRNA/母犬/生殖轴Key words
Nesfatin-1/NUCB2 mRNA/female dog/reproductive axis分类
农业科技引用本文复制引用
蒋书东,张兴旺,王登峰,阎雪松,周伟娟,朱晖,方富贵..Nesfatin-1/NUCB2在犬下丘脑-垂体-卵巢轴相关组织上表达与分布的研究[J].中国兽医科学,2017,47(11):1461-1467,7.基金项目
国家自然科学基金资助项目(31472096) （31472096）
