中国现代医学杂志2017，Vol.27Issue(29)：11-15,5.DOI:10.3969/j.issn.1005-8982.2017.29.003
Bcl-2基因沉默对胃腺癌SGC-7901细胞5-Fu敏感性的影响
Impact of BCL2 gene silencing on 5-Fu sensitivity in gastric adenocarcinoma SGC-7901 cells
摘要
Abstract
Objective To explore the value of B cell lymphoma 2 (BCL2) gene silencing in enhancing 5-fluorouracil (5-FU) sensitivity in gastric adenocarcinoma SGC-7901 cells. Methods Human gastric adenocarcinoma SGC-7901 cells were set up as research group and control group. In the research group, chemically-synthesized BCL2 siRNA sequence was transfected into gastric adenocarcinoma SGC-7901 cells by liposome method;while the control group had no relative intervention. RT-PCR was applied in detection of BCL2 mRNA expression in gastric adenocarcinoma SGC-7901 cells of the two groups before and after transfection. Both groups were added with different concentrations of 5-FU and tagged with Annexin V; 48 h after adding 5-FU flow cytometry was applied to detect cell proliferation inhibition rate and apoptosis rate. Results Before transfection the relative expression of BCL2 mRNA had no statistical difference between the two groups (P > 0.05). The relative expression of BCL2 mRNA in the research group decreased after transfection compared to that before transfection and that in the control group (P < 0.05). After cell culture for 48 h, the proliferation inhibition and apoptosis rates of the research group were higher than those of the control group (P < 0.05). Conclusions BCL2 gene silencing can enhance 5-FU sensitivity in gastric adenocarcinoma SGC-7901 cells, inhibit cancer cell proliferation and promote cancer cell apoptosis; therefore, BCL2 gene silencing may be an effective way to enhance the effect of 5-FU therapy for gastric adenocarcinoma.关键词
B淋巴细胞瘤-2/基因沉默/增强/胃腺癌/SGC-7901细胞/5-氟尿嘧啶/敏感性Key words
B cell lymphoma 2/gene silencing/enhance/gastric adenocarcinoma/SGC-7901 cell/5-fluorouracil/sensitivity分类
医药卫生引用本文复制引用
丁哲宇,杜锋,王继红,甘晟,寸英丽..Bcl-2基因沉默对胃腺癌SGC-7901细胞5-Fu敏感性的影响[J].中国现代医学杂志,2017,27(29):11-15,5.基金项目
昆医联合专项(No :2010CD179) （No :2010CD179）
