中国现代医学杂志2017，Vol.27Issue(29)：32-38,7.DOI:10.3969/j.issn.1005-8982.2017.29.007
晚期非小细胞肺癌外周血CTC与肿瘤组织EGFR表达的相关性
Correlation between EGFR expression in circulating tumor cells and in tumor tissues of advanced non-small cell lung cancer
摘要
Abstract
Objective To detect the expression level of epidermal growth factor receptor (EGFR) in circulating tumor cells (CTC) and matched tumor tissues from patients with advanced non-small cell lung cancer (NSCLC), and investigate the consisitent rate of these two methods. Methods The EGFR expression was analyzed by flow cytometry and immunohistochemical staining in the peripheral blood CTC and tumor tissues from 66 advanced NSCLC patients. The therapeutic efficacy was evaluated after 1 month of treatment with EGFR-tyrosine kinase inhibitor (EGFR-TKI). The consistency of the EGFR expression in the CTC and the tumor tissues was compared. The relationships of EGFR expression with clinical characteristics, efficacy, progression-free survival (PFS) and overall survival (OS) were analyzed. Results The consistent rate of the two methods was 69.7% (46/66, P < 0.05). The EGFR expression levels in the CTC and the tumor tissues were significantly different between the patients with different pathological types (P < 0.05), but not statistically different between the patients with different gender, age, clinical stage or PS score (P > 0.05). The patients with high EGFR expression level had remarkably higher disease control rate than those with low EGFR expression level (CTC: 87.5% vs. 35.3%; tumor tissue: 72.7% vs. 36.4%;P < 0.05), longer PFS (CTC: 8 months vs. 2 months; tumor tissue: 6 months vs. 2 months;P < 0.05) and OS (CTC: 20 months vs. 13 months; tumor tissue: 16 months vs. 14 months; P < 0.05). Conclusions In the patients with advanced NSCLC, EGFR expression level in the CTC and the tumor tissues is highly consistent. Both of them can be used for predicting the efficacy of EGFR-TKI therapy and prognosis. CTC can be a substitute for tumor tissue.关键词
非小细胞肺癌/循环肿瘤细胞/肿瘤组织/表皮生长因子受体Key words
non-small cell lung cancer/circulating tumor cell/tumor tissue/epidermal growth factor receptor分类
医药卫生引用本文复制引用
朱颖,江波,杨承纲,金从国,涂长玲,何文杰,刘坤..晚期非小细胞肺癌外周血CTC与肿瘤组织EGFR表达的相关性[J].中国现代医学杂志,2017,27(29):32-38,7.基金项目
云南省科技厅应用基础研究面上项目(No :2012FB164) （No :2012FB164）
云南省教育厅重点项目(No :2013Z113) （No :2013Z113）
