中国药房2017，Vol.28Issue(34)：4780-4783,4.DOI:10.6039/j.issn.1001-0408.2017.34.07
温阳振衰颗粒对慢性心衰模型大鼠心肌中MEF2表达的影响
Effect of Wenyang Zhenshuai Granules on the Expression of Myocardial MEF2 in Model Rats with Chron-ic Heart Failure
摘要
Abstract
OBJECTIVE:To study the effect of Wenyang zhenshuai granules on myocardial MEF2 in model rats with chronic heart failure(CHF). METHODS:10 rats were randomly selected in 60 rats as normal control group(normal saline),the remain-ing 50 rats were randomly divided into model control group (normal saline),positive control group [enalapril,1.8 mg/(kg·d)], Wenyang zhenshuai granules low-dose,medium-dose,high-dose groups [0.72,1.44,2.88 g/(kg·d)] after induced to CHF models by adriamycin,intragastrically administrated twice a day,for 4 weeks. After administration,heart function indexes [left ventricular ejection fraction (LVEF),left ventricular fractional shortening (LVFS),left ventricular end-diastolic diameter (LVSD) and left ventricular end-diastolic diameter(LVDD)] of rats in each group were detected,protein expressions of myocardial MEF2 and phos-phorylated MEF2 (p-MEF2) and mRNA expression of MEF2 were determined. RESULTS:Compared with normal control group, LVEF,LVFS of rats in model control group and each administration group were significantly reduced (P<0.05),LVSD,LVDD were significantly increased (P<0.05);protein expression of myocardial p-MEF2 was significantly down-regulated (P<0.05), and there were no statistical significances in protein or mRNA expressions of MEF2 (P>0.05). Compared with model control group,heart function indexes of rats in each administration group were significantly improved (P<0.05);protein expression of myocardial p-MEF2 was significantly up-regulated (P<0.05),and there were no statistical significances in protein or mRNA ex-pressions of MEF2(P>0.05). CONCLUSIONS:Wenyang zhenshuai granule can antagonize the down-regulation effect of adriamy-cin-induced MEF2 protein phosphorylation,which may be one of the mechanisms of its treatment for CHF.关键词
温阳振衰颗粒/慢性心衰/心肌细胞增强子/磷酸化心肌细胞增强子/大鼠Key words
Wenyang zhenshuai granules/Chronic heart failure/Myocardial cell enhancer/Phosphorylated myocardial cell en-hancer/Rats分类
医药卫生引用本文复制引用
蔡虎志,王笑莹,陈青扬,陈新宇..温阳振衰颗粒对慢性心衰模型大鼠心肌中MEF2表达的影响[J].中国药房,2017,28(34):4780-4783,4.基金项目
国家自然科学基金资助项目(No.81173213、81704-061) （No.81173213、81704-061）
湖南省教育厅资助项目(No.15C1047) （No.15C1047）
2016年度湖南省中医药科研计划项目(No.201651) （No.201651）
