中国药房2017，Vol.28Issue(34)：4784-4787,4.DOI:10.6039/j.issn.1001-0408.2017.34.08
二氢杨梅素对高糖诱导的肾小球系膜细胞增殖及纤维连接蛋白堆积的影响
Effect of Dihydromyricetin on High Glucose-induced Glomerular Mesangial Cell Proliferation and Fibro-nectin Accumulation
摘要
Abstract
OBJECTIVE:To study the effect of dihydromyricetin(DMY)on high glucose(HG)-induced glomerular mesangial cell(MCs)proliferation and fibronectin(FN)accumulation,and explore its mechanism for diabetic nephropathy glomerulosclero-sis. METHODS:Cells were divided into normal group(5.5 mmol/L glucose),HG group(30 mmol/L glucose),DMY low-concen-tration,medium-concentration,high-concentration groups (30 mmol/L glucose+22.5,45,90 μmol/L DMY). After incubating 48 h,MTT was used to detect the proliferative activity [reflected by the optical density(OD)value] of cells;molecular docking meth-od was adopted to conduct simulation analysis for DMY binding state with Smad2. Cells were divided into normal group(5.5 mmol/L glucose),HG group (30 mmol/L glucose),DMY group (30 mmol/L glucose+45 μmol/L DMY) and DMY control group (5.5 mmol/L glucose+45 μmol/L DMY). After incubating 5 h,Western blot was used to detect the expression levels of phosphorylated Smad2 (p-Smad2) and extracellular matrix protein FN. RESULTS:Results of MTT detection showed,compared with normal group,OD values in HG group were significantly increased(P<0.05);compared with HG group,OD values in DMY each con-centration group were significantly reduced(P<0.05). The Gibbs free energy(ΔG)of DMY and Smad2 protein was -5.64 kJ/mol, Ki was 73.53 μmol/L,and there were hydrogen bond donor and receptor binding in No. 465,464,461,458 amino acid residues. Results of Western blot showed,compared with normal group,expression levels of p-Smad2 and extracellular matrix protein FN in HG group were significantly increased (P<0.05);compared with HG group,expression levels of p-Smad2 and extracellular ma-trix protein FN in DMY group were significantly decreased(P<0.05). CONCLUSIONS:DMY inhibits HG-induced MCs prolifera-tion and improves diabetic nephropathy glomerulosclerosis by combining with Smad2 and inhibiting Smad2 phosphorylation to re-duce the extracellular matrix protein FN expression.关键词
二氢杨梅素/肾小球系膜细胞/增殖/纤维连接蛋白/分子对接Key words
Dihydromyricetin/Glomerular mesangial cells/Proliferation/Fibronectin/Molecular docking分类
医药卫生引用本文复制引用
李加林,郭小华,吴艳娇,黄志伟,刘思齐,吴素珍..二氢杨梅素对高糖诱导的肾小球系膜细胞增殖及纤维连接蛋白堆积的影响[J].中国药房,2017,28(34):4784-4787,4.基金项目
国家自然科学基金资助项目(No.31660334) （No.31660334）
江西省青年科学基金资助项目(No.20161BAB215217) （No.20161BAB215217）
